Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

Emmanuel Scorsone; Raafa Manai; Maria J Ricatti; Marco Redaelli; Philippe Bergonzo; Krishna C Persaud; Carla Mucignat

doi:10.1109/JSEN.2016.2585187

Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

Emmanuel Scorsone
, Raafa Manai
, Maria J Ricatti
, Marco Redaelli
, Philippe Bergonzo
, Krishna C Persaud
, Carla Mucignat

Research output: Contribution to journal › Article › peer-review

59 Downloads (Pure)

Abstract

A new bio-sensing platform based on major urinary
proteins (MUPs) from the mouse as chemical recognition
elements has been developed. The transducers were surface
acoustic devices coated with diamond nanoparticles as an intermediate
layer enabling covalent attachment of the proteins.
The resulting sensors detected 2,4-Dinitrotoluene, 4-Nitrotoluene,
and 2-Isobutyl-3-methoxypyrazine at ppb levels. The best sensor
showed a sensitivity of 24 000 Hz · ppm−1 to 2, 4-DNT when
grafted with the protein MUP20. Trends in the sensitivity of
the various VOC sensors were compared with the association
constant values Ka of the proteins to target ligands measured by
competitive assay in liquid phase. The system is able to detect
analytes both in liquid as well as vapor phase and indicate that
MUPs are robust bio-recognition elements that can be utilized in
artificial olfaction applications.

Original language	Undefined
Pages (from-to)	6543-6550
Number of pages	8
Journal	IEEE Sensors Journal
Volume	16
Issue number	17
Early online date	27 Jun 2016
DOIs	https://doi.org/10.1109/JSEN.2016.2585187
Publication status	Published - 3 Aug 2016

Keywords

Nanodiamond, major urinary proteins, surface acoustic wave sensors, artificial olfaction.

Access to Document

10.1109/JSEN.2016.2585187

Scorsone-et-al vReviewIEEE v2 AAMAccepted author manuscript, 1.42 MB

Cite this

@article{c0e4d5da1b924e98b17457ba10a45921,

title = "Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction",

abstract = "A new bio-sensing platform based on major urinary proteins (MUPs) from the mouse as chemical recognition elements has been developed. The transducers were surface acoustic devices coated with diamond nanoparticles as an intermediate layer enabling covalent attachment of the proteins. The resulting sensors detected 2,4-Dinitrotoluene, 4-Nitrotoluene, and 2-Isobutyl-3-methoxypyrazine at ppb levels. The best sensor showed a sensitivity of 24 000 Hz · ppm−1 to 2, 4-DNT when grafted with the protein MUP20. Trends in the sensitivity of the various VOC sensors were compared with the association constant values Ka of the proteins to target ligands measured by competitive assay in liquid phase. The system is able to detect analytes both in liquid as well as vapor phase and indicate that MUPs are robust bio-recognition elements that can be utilized in artificial olfaction applications.",

keywords = "Nanodiamond, major urinary proteins, surface acoustic wave sensors, artificial olfaction.",

author = "Emmanuel Scorsone and Raafa Manai and Ricatti, \{Maria J\} and Marco Redaelli and Philippe Bergonzo and Persaud, \{Krishna C\} and Carla Mucignat",

year = "2016",

month = aug,

day = "3",

doi = "10.1109/JSEN.2016.2585187",

language = "Undefined",

volume = "16",

pages = "6543--6550",

journal = "IEEE Sensors Journal",

issn = "2379-9153",

publisher = "IEEE",

number = "17",

}

TY - JOUR

T1 - Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

AU - Scorsone, Emmanuel

AU - Manai, Raafa

AU - Ricatti, Maria J

AU - Redaelli, Marco

AU - Bergonzo, Philippe

AU - Persaud, Krishna C

AU - Mucignat, Carla

PY - 2016/8/3

Y1 - 2016/8/3

N2 - A new bio-sensing platform based on major urinary proteins (MUPs) from the mouse as chemical recognition elements has been developed. The transducers were surface acoustic devices coated with diamond nanoparticles as an intermediate layer enabling covalent attachment of the proteins. The resulting sensors detected 2,4-Dinitrotoluene, 4-Nitrotoluene, and 2-Isobutyl-3-methoxypyrazine at ppb levels. The best sensor showed a sensitivity of 24 000 Hz · ppm−1 to 2, 4-DNT when grafted with the protein MUP20. Trends in the sensitivity of the various VOC sensors were compared with the association constant values Ka of the proteins to target ligands measured by competitive assay in liquid phase. The system is able to detect analytes both in liquid as well as vapor phase and indicate that MUPs are robust bio-recognition elements that can be utilized in artificial olfaction applications.

AB - A new bio-sensing platform based on major urinary proteins (MUPs) from the mouse as chemical recognition elements has been developed. The transducers were surface acoustic devices coated with diamond nanoparticles as an intermediate layer enabling covalent attachment of the proteins. The resulting sensors detected 2,4-Dinitrotoluene, 4-Nitrotoluene, and 2-Isobutyl-3-methoxypyrazine at ppb levels. The best sensor showed a sensitivity of 24 000 Hz · ppm−1 to 2, 4-DNT when grafted with the protein MUP20. Trends in the sensitivity of the various VOC sensors were compared with the association constant values Ka of the proteins to target ligands measured by competitive assay in liquid phase. The system is able to detect analytes both in liquid as well as vapor phase and indicate that MUPs are robust bio-recognition elements that can be utilized in artificial olfaction applications.

KW - Nanodiamond, major urinary proteins, surface acoustic wave sensors, artificial olfaction.

UR - https://www.scopus.com/pages/publications/84982095352

U2 - 10.1109/JSEN.2016.2585187

DO - 10.1109/JSEN.2016.2585187

M3 - Article

SN - 2379-9153

VL - 16

SP - 6543

EP - 6550

JO - IEEE Sensors Journal

JF - IEEE Sensors Journal

IS - 17

ER -

Major Urinary Proteins on Nanodiamond-Based Resonators Toward Artificial Olfaction

Abstract

Keywords

Access to Document

Other files and links

Cite this