Mapping of the human complement factor I gene to 4q25

R Shiang; J C Murray; C C Morton; K H Buetow; J J Wasmuth; A H Olney; W G Sanger; G Goldberger

Mapping of the human complement factor I gene to 4q25

R Shiang
, J C Murray
, C C Morton
, K H Buetow
, J J Wasmuth
, A H Olney
, W G Sanger
, G Goldberger

Division of Evolution, Infection and Genomics

The University of Iowa

Research output: Contribution to journal › Article › peer-review

Abstract

A detailed genetic and physical map of human complement factor I (IF) using somatic cell hybrids, in situ hybridization, and genetic linkage is reported. The gene has been localized to band 4q25. The order GC-INP10-ADH3-EGF-IF-IL2-MNS is proposed for genes on 4q on the basis of genetic and physical mapping techniques. A BclI polymorphism found with the IF probe demonstrated a maternal origin for a de novo deletion of chromosome 4 that was used in physically mapping the gene. The genetic and physical distances around band 4q24 suggest that 1 cM is approximately 1.2 million bp of DNA. This work provides a useful addition to the map of 4q.

Original language	English
Pages (from-to)	82-6
Number of pages	5
Journal	Genomics
Volume	4
Issue number	1
Publication status	Published - Jan 1989

Keywords

Chromosome Mapping
Chromosomes, Human, Pair 4
Complement Factor I
Genetic Linkage
Humans
Hybrid Cells
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
Serine Endopeptidases
Journal Article
Research Support, U.S. Gov't, P.H.S.

Cite this

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title = "Mapping of the human complement factor I gene to 4q25",

abstract = "A detailed genetic and physical map of human complement factor I (IF) using somatic cell hybrids, in situ hybridization, and genetic linkage is reported. The gene has been localized to band 4q25. The order GC-INP10-ADH3-EGF-IF-IL2-MNS is proposed for genes on 4q on the basis of genetic and physical mapping techniques. A BclI polymorphism found with the IF probe demonstrated a maternal origin for a de novo deletion of chromosome 4 that was used in physically mapping the gene. The genetic and physical distances around band 4q24 suggest that 1 cM is approximately 1.2 million bp of DNA. This work provides a useful addition to the map of 4q.",

keywords = "Chromosome Mapping, Chromosomes, Human, Pair 4, Complement Factor I, Genetic Linkage, Humans, Hybrid Cells, Nucleic Acid Hybridization, Polymorphism, Restriction Fragment Length, Serine Endopeptidases, Journal Article, Research Support, U.S. Gov't, P.H.S.",

author = "R Shiang and Murray, \{J C\} and Morton, \{C C\} and Buetow, \{K H\} and Wasmuth, \{J J\} and Olney, \{A H\} and Sanger, \{W G\} and G Goldberger",

year = "1989",

month = jan,

language = "English",

volume = "4",

pages = "82--6",

journal = "Genomics",

issn = "0888-7543",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - Mapping of the human complement factor I gene to 4q25

AU - Shiang, R

AU - Murray, J C

AU - Morton, C C

AU - Buetow, K H

AU - Wasmuth, J J

AU - Olney, A H

AU - Sanger, W G

AU - Goldberger, G

PY - 1989/1

Y1 - 1989/1

N2 - A detailed genetic and physical map of human complement factor I (IF) using somatic cell hybrids, in situ hybridization, and genetic linkage is reported. The gene has been localized to band 4q25. The order GC-INP10-ADH3-EGF-IF-IL2-MNS is proposed for genes on 4q on the basis of genetic and physical mapping techniques. A BclI polymorphism found with the IF probe demonstrated a maternal origin for a de novo deletion of chromosome 4 that was used in physically mapping the gene. The genetic and physical distances around band 4q24 suggest that 1 cM is approximately 1.2 million bp of DNA. This work provides a useful addition to the map of 4q.

AB - A detailed genetic and physical map of human complement factor I (IF) using somatic cell hybrids, in situ hybridization, and genetic linkage is reported. The gene has been localized to band 4q25. The order GC-INP10-ADH3-EGF-IF-IL2-MNS is proposed for genes on 4q on the basis of genetic and physical mapping techniques. A BclI polymorphism found with the IF probe demonstrated a maternal origin for a de novo deletion of chromosome 4 that was used in physically mapping the gene. The genetic and physical distances around band 4q24 suggest that 1 cM is approximately 1.2 million bp of DNA. This work provides a useful addition to the map of 4q.

KW - Chromosome Mapping

KW - Chromosomes, Human, Pair 4

KW - Complement Factor I

KW - Genetic Linkage

KW - Humans

KW - Hybrid Cells

KW - Nucleic Acid Hybridization

KW - Polymorphism, Restriction Fragment Length

KW - Serine Endopeptidases

KW - Journal Article

KW - Research Support, U.S. Gov't, P.H.S.

M3 - Article

C2 - 2563353

SN - 0888-7543

VL - 4

SP - 82

EP - 86

JO - Genomics

JF - Genomics

IS - 1

ER -

Mapping of the human complement factor I gene to 4q25

Abstract

Keywords

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