Mass-mapping of ECM macromolecules by scanning transmission electron microscopy.

Michael J. Sherratt, Helen K. Graham, Cay M. Kielty, David F. Holmes

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In the scanning transmission electron microscope, the degree of electron scattering induced by biological specimens, such as ECM macromolecules, is dependent on the molecular mass. By calibrating the ratio of scattered to non-scattered electrons against a known mass standard, such as tobacco mosaic virus, it is possible to quantify absolute changes in both mass and mass distribution. These mass mapping approaches can provide important information on ECM assembly, organisation, and interactions which is not obtainable by other means.
    Original languageEnglish
    Pages (from-to)151-161
    Number of pages10
    JournalMethods in Molecular Biology
    Volume522
    DOIs
    Publication statusPublished - 2009

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