Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes

Matthew J. Baker; Leiliang Zheng; Nicholas Winograd; Nicholas P. Lockyer; John C. Vickerman

doi:10.1021/la802582f

Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes

Matthew J. Baker
, Leiliang Zheng
, Nicholas Winograd
, Nicholas P. Lockyer
, John C. Vickerman

Research output: Contribution to journal › Article › peer-review

Abstract

Time of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir-Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid-protein and lipid-lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/ glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images. © 2008 American Chemical Society.

Original language	English
Pages (from-to)	11803-11810
Number of pages	7
Journal	Langmuir
Volume	24
Issue number	20
DOIs	https://doi.org/10.1021/la802582f
Publication status	Published - 21 Oct 2008

Keywords

Glycophorins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (glycophorin A
mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes)
Cell membrane
Heterogeneity
Langmuir-Blodgett films
Membrane
Principal component analysis
TOF-SIMS (mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes)
Glycophospholipids Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes)
Imaging (mass spectrometric
glycophospholipid mass spectral imaging model cell membrane
cholesterol mass spectral imaging model cell membrane
glycophorin A mass spectral imaging model cell membrane

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10.1021/la802582f

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title = "Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes",

abstract = "Time of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir-Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid-protein and lipid-lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/ glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images. {\textcopyright} 2008 American Chemical Society.",

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author = "Baker, \{Matthew J.\} and Leiliang Zheng and Nicholas Winograd and Lockyer, \{Nicholas P.\} and Vickerman, \{John C.\}",

year = "2008",

month = oct,

day = "21",

doi = "10.1021/la802582f",

language = "English",

volume = "24",

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journal = "Langmuir",

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publisher = "American Chemical Society",

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T1 - Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes

AU - Baker, Matthew J.

AU - Zheng, Leiliang

AU - Winograd, Nicholas

AU - Lockyer, Nicholas P.

AU - Vickerman, John C.

PY - 2008/10/21

Y1 - 2008/10/21

N2 - Time of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir-Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid-protein and lipid-lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/ glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images. © 2008 American Chemical Society.

AB - Time of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir-Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid-protein and lipid-lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/ glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images. © 2008 American Chemical Society.

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KW - Cell membrane

KW - Heterogeneity

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KW - Principal component analysis

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KW - Glycophospholipids Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes)

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KW - cholesterol mass spectral imaging model cell membrane

KW - glycophorin A mass spectral imaging model cell membrane

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Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin A in model cell membranes

Abstract

Keywords

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