TY - JOUR
T1 - Mass Spectrometry‐Based Abundance Atlas of ABC Transporters in Human Liver, Gut, Kidney, Brain, and Skin
AU - Al-Majdoub, Zubida
AU - Achour, Brahim
AU - Couto, Narciso
AU - Howard, Martyn
AU - Elmorsi, Yasmine
AU - Scotcher, Daniel
AU - Alrubia, Sarah
AU - Elkhateeb, Eman
AU - Vasilogianni, Areti Maria
AU - Alohali, Noura
AU - Neuhoff, Sibylle
AU - Schmidt, Lutz
AU - Rostami-Hodjegan, Amin
AU - Barber, Jill
PY - 2020/10/31
Y1 - 2020/10/31
N2 - ABC transporters traffic drugs and their metabolites across membranes, making ABC‐transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low‐abundance transmembrane proteins. Here we analyzed 200 samples of crude and membrane‐enriched fractions from human liver, kidney, intestine, brain microvessels, and skin, by label‐free quantitative mass spectrometry. We identified 32 (out of 48) ABC transporters: ABCD3 was the most abundant in liver, whereas ABCA8, ABCB2/TAP1, and ABCE1 were detected in all tissues. Interestingly, this atlas unveiled that ABCB2/TAP1 may have TAP2‐independent functions in the brain, and that biliary atresia and control livers have quite different ABC‐transporter profiles. We propose that meaningful biological information can be derived from a direct comparison of these datasets.
AB - ABC transporters traffic drugs and their metabolites across membranes, making ABC‐transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low‐abundance transmembrane proteins. Here we analyzed 200 samples of crude and membrane‐enriched fractions from human liver, kidney, intestine, brain microvessels, and skin, by label‐free quantitative mass spectrometry. We identified 32 (out of 48) ABC transporters: ABCD3 was the most abundant in liver, whereas ABCA8, ABCB2/TAP1, and ABCE1 were detected in all tissues. Interestingly, this atlas unveiled that ABCB2/TAP1 may have TAP2‐independent functions in the brain, and that biliary atresia and control livers have quite different ABC‐transporter profiles. We propose that meaningful biological information can be derived from a direct comparison of these datasets.
U2 - 10.1002/1873-3468.13982
DO - 10.1002/1873-3468.13982
M3 - Article
SN - 1873-3468
JO - FEBS Letters
JF - FEBS Letters
ER -