Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

Ann E. Canfield; Mary Jo Doherty; Victoria Kelly; Bill Newman; Claire Farrington; Michael E. Grant; Raymond P. Boot-Handford

doi:10.1016/S0014-5793(00)02363-2

Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

Ann E. Canfield, Mary Jo Doherty, Victoria Kelly, Bill Newman, Claire Farrington, Michael E. Grant, Raymond P. Boot-Handford

Research output: Contribution to journal › Article › peer-review

Abstract

PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification. © 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	267-271
Number of pages	4
Journal	FEBS Letters
Volume	487
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(00)02363-2
Publication status	Published - 29 Dec 2000

Keywords

Calcification
Matrix Gla protein
Pericyte

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title = "Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes",

abstract = "PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification. {\textcopyright} 2000 Federation of European Biochemical Societies.",

keywords = "Calcification, Matrix Gla protein, Pericyte",

author = "Canfield, {Ann E.} and Doherty, {Mary Jo} and Victoria Kelly and Bill Newman and Claire Farrington and Grant, {Michael E.} and Boot-Handford, {Raymond P.}",

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doi = "10.1016/S0014-5793(00)02363-2",

language = "English",

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T1 - Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

AU - Canfield, Ann E.

AU - Doherty, Mary Jo

AU - Kelly, Victoria

AU - Newman, Bill

AU - Farrington, Claire

AU - Grant, Michael E.

AU - Boot-Handford, Raymond P.

PY - 2000/12/29

Y1 - 2000/12/29

N2 - PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification. © 2000 Federation of European Biochemical Societies.

AB - PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification. © 2000 Federation of European Biochemical Societies.

KW - Calcification

KW - Matrix Gla protein

KW - Pericyte

U2 - 10.1016/S0014-5793(00)02363-2

DO - 10.1016/S0014-5793(00)02363-2

M3 - Article

C2 - 11150522

VL - 487

SP - 267

EP - 271

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

Abstract

Keywords

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