Measurement of plasma membrane calcium-calmodulin-dependent ATPase (PMCA) activity.

Tamer M A Mohamed; Florence M. Baudoin-Stanley; Riham Abou-Leisa; Elizabeth Cartwright; Ludwig Neyses; Delvac Oceandy

doi:10.1007/978-1-60761-700-6_18

Measurement of plasma membrane calcium-calmodulin-dependent ATPase (PMCA) activity.

Tamer M A Mohamed, Florence M. Baudoin-Stanley, Riham Abou-Leisa, Elizabeth Cartwright, Ludwig Neyses, Delvac Oceandy

Research output: Chapter in Book/Conference proceeding › Chapter

Abstract

The plasma membrane calcium-calmodulin-dependent ATPase (PMCA) is a calcium-extruding enzymatic pump that ejects calcium from the cytoplasm to the extracellular compartment. Although in excitable cells such as skeletal and cardiac muscle cells PMCA has been shown to play only a minor role in regulating global intracellular calcium concentration, increasing evidence points to an important role for PMCA in signal transduction, in particular in the nitric oxide signaling pathway. Moreover, recent evidence has shown the functional importance of PMCA in mediating cardiac contractility and vascular tone. Here we describe a method in determining PMCA activity in the microsomal membrane preparation from cultured cells that overexpress specific isoform of PMCA by using modified coupled enzyme assay.

Original language	English
Title of host publication	Methods in molecular biology (Clifton, N.J.)\|Methods Mol. Biol.
Subtitle of host publication	Methods and Protocols
Place of Publication	New York
Publisher	Springer Nature
Pages	333-342
Number of pages	9
Volume	637
DOIs	https://doi.org/10.1007/978-1-60761-700-6_18
Publication status	Published - 2010

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10.1007/978-1-60761-700-6_18

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title = "Measurement of plasma membrane calcium-calmodulin-dependent ATPase (PMCA) activity.",

abstract = "The plasma membrane calcium-calmodulin-dependent ATPase (PMCA) is a calcium-extruding enzymatic pump that ejects calcium from the cytoplasm to the extracellular compartment. Although in excitable cells such as skeletal and cardiac muscle cells PMCA has been shown to play only a minor role in regulating global intracellular calcium concentration, increasing evidence points to an important role for PMCA in signal transduction, in particular in the nitric oxide signaling pathway. Moreover, recent evidence has shown the functional importance of PMCA in mediating cardiac contractility and vascular tone. Here we describe a method in determining PMCA activity in the microsomal membrane preparation from cultured cells that overexpress specific isoform of PMCA by using modified coupled enzyme assay.",

author = "Mohamed, {Tamer M A} and Baudoin-Stanley, {Florence M.} and Riham Abou-Leisa and Elizabeth Cartwright and Ludwig Neyses and Delvac Oceandy",

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doi = "10.1007/978-1-60761-700-6_18",

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T1 - Measurement of plasma membrane calcium-calmodulin-dependent ATPase (PMCA) activity.

AU - Mohamed, Tamer M A

AU - Baudoin-Stanley, Florence M.

AU - Abou-Leisa, Riham

AU - Cartwright, Elizabeth

AU - Neyses, Ludwig

AU - Oceandy, Delvac

PY - 2010

Y1 - 2010

N2 - The plasma membrane calcium-calmodulin-dependent ATPase (PMCA) is a calcium-extruding enzymatic pump that ejects calcium from the cytoplasm to the extracellular compartment. Although in excitable cells such as skeletal and cardiac muscle cells PMCA has been shown to play only a minor role in regulating global intracellular calcium concentration, increasing evidence points to an important role for PMCA in signal transduction, in particular in the nitric oxide signaling pathway. Moreover, recent evidence has shown the functional importance of PMCA in mediating cardiac contractility and vascular tone. Here we describe a method in determining PMCA activity in the microsomal membrane preparation from cultured cells that overexpress specific isoform of PMCA by using modified coupled enzyme assay.

AB - The plasma membrane calcium-calmodulin-dependent ATPase (PMCA) is a calcium-extruding enzymatic pump that ejects calcium from the cytoplasm to the extracellular compartment. Although in excitable cells such as skeletal and cardiac muscle cells PMCA has been shown to play only a minor role in regulating global intracellular calcium concentration, increasing evidence points to an important role for PMCA in signal transduction, in particular in the nitric oxide signaling pathway. Moreover, recent evidence has shown the functional importance of PMCA in mediating cardiac contractility and vascular tone. Here we describe a method in determining PMCA activity in the microsomal membrane preparation from cultured cells that overexpress specific isoform of PMCA by using modified coupled enzyme assay.

U2 - 10.1007/978-1-60761-700-6_18

DO - 10.1007/978-1-60761-700-6_18

M3 - Chapter

VL - 637

SP - 333

EP - 342

BT - Methods in molecular biology (Clifton, N.J.)|Methods Mol. Biol.

PB - Springer Nature

CY - New York

ER -

Measurement of plasma membrane calcium-calmodulin-dependent ATPase (PMCA) activity.

Abstract

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