TY - JOUR
T1 - Measurement of plasma membrane calcium-calmodulin-dependent ATPase (PMCA) activity.
AU - Mohamed, Tamer M A
AU - Baudoin-Stanley, Florence M.
AU - Abou-Leisa, Riham
AU - Cartwright, Elizabeth
AU - Neyses, Ludwig
AU - Oceandy, Delvac
PY - 2010
Y1 - 2010
N2 - The plasma membrane calcium-calmodulin-dependent ATPase (PMCA) is a calcium-extruding enzymatic pump that ejects calcium from the cytoplasm to the extracellular compartment. Although in excitable cells such as skeletal and cardiac muscle cells PMCA has been shown to play only a minor role in regulating global intracellular calcium concentration, increasing evidence points to an important role for PMCA in signal transduction, in particular in the nitric oxide signaling pathway. Moreover, recent evidence has shown the functional importance of PMCA in mediating cardiac contractility and vascular tone. Here we describe a method in determining PMCA activity in the microsomal membrane preparation from cultured cells that overexpress specific isoform of PMCA by using modified coupled enzyme assay.
AB - The plasma membrane calcium-calmodulin-dependent ATPase (PMCA) is a calcium-extruding enzymatic pump that ejects calcium from the cytoplasm to the extracellular compartment. Although in excitable cells such as skeletal and cardiac muscle cells PMCA has been shown to play only a minor role in regulating global intracellular calcium concentration, increasing evidence points to an important role for PMCA in signal transduction, in particular in the nitric oxide signaling pathway. Moreover, recent evidence has shown the functional importance of PMCA in mediating cardiac contractility and vascular tone. Here we describe a method in determining PMCA activity in the microsomal membrane preparation from cultured cells that overexpress specific isoform of PMCA by using modified coupled enzyme assay.
U2 - 10.1007/978-1-60761-700-6_18
DO - 10.1007/978-1-60761-700-6_18
M3 - Article
C2 - 20419444
SN - 1064-3745
VL - 637
SP - 333
EP - 342
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -