Mechanical removal of dendritic cell-generating non-classical monocytes via ex vivo lung perfusion

James Fildes; John P. Stone; Hannah Sevenoaks; Trygve Sjöberg; Stig Steen; Nizar Yonan; James E. Fildes

doi:10.1016/j.healun.2014.03.005

Mechanical removal of dendritic cell-generating non-classical monocytes via ex vivo lung perfusion

James Fildes, John P. Stone, Hannah Sevenoaks, Trygve Sjöberg, Stig Steen, Nizar Yonan, James E. Fildes

Research output: Contribution to journal › Article › peer-review

Abstract

Background Ex vivo lung perfusion (EVLP) is a novel procedure designed to rapidly assess and recondition unusable donor lungs for transplantation (LTx). EVLP may reduce graft immunogenicity and allorecognition via removal of passenger leukocytes. We aimed to explore this hypothesis using human EVLP and in vitro analysis. Methods Explanted human lungs (n = 7) underwent standard EVLP. Perfusate samples and the leukocyte filter were collected, and cells characterized via flow cytometry. Isolated alveolar monocytes (from post-LTx bronchoalveolar lavage) were differentiated to dendritic cells and characterized (n = 10). An in vitro (air epithelial-liquid endothelial) lung model was utilized to evaluate monocyte migration and differentiation within the lung. Results Non-classical monocytes (NCM, normally

Original language	English
Pages (from-to)	864-869
Number of pages	5
Journal	Journal of Heart and Lung Transplantation
Volume	33
Issue number	8
DOIs	https://doi.org/10.1016/j.healun.2014.03.005
Publication status	Published - 2014

Keywords

dendritic cell
diapedesis
ex vivo lung perfusion
lung transplantation
monocyte
non-classical monocyte
passenger leukocytes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.healun.2014.03.005

http://www.elsevier.com/locate/healun

Cite this

@article{a93947567b37444284cdc99e4e29cb83,

title = "Mechanical removal of dendritic cell-generating non-classical monocytes via ex vivo lung perfusion",

abstract = "Background Ex vivo lung perfusion (EVLP) is a novel procedure designed to rapidly assess and recondition unusable donor lungs for transplantation (LTx). EVLP may reduce graft immunogenicity and allorecognition via removal of passenger leukocytes. We aimed to explore this hypothesis using human EVLP and in vitro analysis. Methods Explanted human lungs (n = 7) underwent standard EVLP. Perfusate samples and the leukocyte filter were collected, and cells characterized via flow cytometry. Isolated alveolar monocytes (from post-LTx bronchoalveolar lavage) were differentiated to dendritic cells and characterized (n = 10). An in vitro (air epithelial-liquid endothelial) lung model was utilized to evaluate monocyte migration and differentiation within the lung. Results Non-classical monocytes (NCM, normally",

keywords = "dendritic cell, diapedesis, ex vivo lung perfusion, lung transplantation, monocyte, non-classical monocyte, passenger leukocytes",

author = "James Fildes and Stone, {John P.} and Hannah Sevenoaks and Trygve Sj{\"o}berg and Stig Steen and Nizar Yonan and Fildes, {James E.}",

year = "2014",

doi = "10.1016/j.healun.2014.03.005",

language = "English",

volume = "33",

pages = "864--869",

journal = "Journal of Heart and Lung Transplantation",

issn = "1053-2498",

publisher = "Elsevier BV",

number = "8",

}

TY - JOUR

T1 - Mechanical removal of dendritic cell-generating non-classical monocytes via ex vivo lung perfusion

AU - Fildes, James

AU - Stone, John P.

AU - Sevenoaks, Hannah

AU - Sjöberg, Trygve

AU - Steen, Stig

AU - Yonan, Nizar

AU - Fildes, James E.

PY - 2014

Y1 - 2014

N2 - Background Ex vivo lung perfusion (EVLP) is a novel procedure designed to rapidly assess and recondition unusable donor lungs for transplantation (LTx). EVLP may reduce graft immunogenicity and allorecognition via removal of passenger leukocytes. We aimed to explore this hypothesis using human EVLP and in vitro analysis. Methods Explanted human lungs (n = 7) underwent standard EVLP. Perfusate samples and the leukocyte filter were collected, and cells characterized via flow cytometry. Isolated alveolar monocytes (from post-LTx bronchoalveolar lavage) were differentiated to dendritic cells and characterized (n = 10). An in vitro (air epithelial-liquid endothelial) lung model was utilized to evaluate monocyte migration and differentiation within the lung. Results Non-classical monocytes (NCM, normally

AB - Background Ex vivo lung perfusion (EVLP) is a novel procedure designed to rapidly assess and recondition unusable donor lungs for transplantation (LTx). EVLP may reduce graft immunogenicity and allorecognition via removal of passenger leukocytes. We aimed to explore this hypothesis using human EVLP and in vitro analysis. Methods Explanted human lungs (n = 7) underwent standard EVLP. Perfusate samples and the leukocyte filter were collected, and cells characterized via flow cytometry. Isolated alveolar monocytes (from post-LTx bronchoalveolar lavage) were differentiated to dendritic cells and characterized (n = 10). An in vitro (air epithelial-liquid endothelial) lung model was utilized to evaluate monocyte migration and differentiation within the lung. Results Non-classical monocytes (NCM, normally

KW - dendritic cell

KW - diapedesis

KW - ex vivo lung perfusion

KW - lung transplantation

KW - monocyte

KW - non-classical monocyte

KW - passenger leukocytes

U2 - 10.1016/j.healun.2014.03.005

DO - 10.1016/j.healun.2014.03.005

M3 - Article

SN - 1053-2498

VL - 33

SP - 864

EP - 869

JO - Journal of Heart and Lung Transplantation

JF - Journal of Heart and Lung Transplantation

IS - 8

ER -

Mechanical removal of dendritic cell-generating non-classical monocytes via ex vivo lung perfusion

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this