Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis

Douglas Kell; Neil Swainston; P Pir; SG Oliver

doi:10.1016/j.tibtech.2015.02.001

Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis

Douglas Kell, Neil Swainston, P Pir, SG Oliver

Research output: Contribution to journal › Article › peer-review

Abstract

Because they mainly do not involve chemical changes, membrane transporters have been a Cinderella subject in the biotechnology of small molecule production, but this is a serious oversight. Influx transporters contribute significantly to the flux towards product, and efflux transporters ensure the accumulation of product in the much greater extracellular space of fermentors. Programmes for improving biotechnological processes might therefore give greater consideration to transporters than may have been commonplace. Strategies for identifying important transporters include expression profiling, genome-wide knockout studies, stress-based selection, and the use of inhibitors. In addition, modern methods of directed evolution and synthetic biology, especially those effecting changes in energy coupling, offer huge opportunities for increasing the flux towards extracellular product formation by transporter engineering.

Original language	English
Pages (from-to)	237-246
Journal	Trends in Biotechnology
Volume	33
Issue number	4
DOIs	https://doi.org/10.1016/j.tibtech.2015.02.001
Publication status	Published - Mar 2015

Keywords

drug transporters; biotechnology; stress tolerance; flux improvements; GWAS

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

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10.1016/j.tibtech.2015.02.001

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals
Scrutton, N. (PI), Azapagic, A. (CoI), Balmer, A. (CoI), Barran, P. (CoI), Breitling, R. (CoI), Delneri, D. (CoI), Dixon, N. (CoI), Faulon, J.-L. (CoI), Flitsch, S. (CoI), Goble, C. (CoI), Goodacre, R. (CoI), Hay, S. (CoI), Kell, D. (CoI), Leys, D. (CoI), Lloyd, J. (CoI), Lockyer, N. (CoI), Martin, P. (CoI), Micklefield, J. (CoI), Munro, A. (CoI), Pedrosa Mendes, P. (CoI), Randles, S. (CoI), Salehi Yazdi, F. (CoI), Shapira, P. (CoI), Takano, E. (CoI), Turner, N. (CoI) & Winterburn, J. (CoI)
14/11/14 → 13/05/20
Project: Research

Cite this

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title = "Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis",

abstract = "Because they mainly do not involve chemical changes, membrane transporters have been a Cinderella subject in the biotechnology of small molecule production, but this is a serious oversight. Influx transporters contribute significantly to the flux towards product, and efflux transporters ensure the accumulation of product in the much greater extracellular space of fermentors. Programmes for improving biotechnological processes might therefore give greater consideration to transporters than may have been commonplace. Strategies for identifying important transporters include expression profiling, genome-wide knockout studies, stress-based selection, and the use of inhibitors. In addition, modern methods of directed evolution and synthetic biology, especially those effecting changes in energy coupling, offer huge opportunities for increasing the flux towards extracellular product formation by transporter engineering.",

keywords = "drug transporters; biotechnology; stress tolerance; flux improvements; GWAS",

author = "Douglas Kell and Neil Swainston and P Pir and SG Oliver",

note = "D.B.K. thanks the Biotechnology and Biological Sciences Research Council (BBSRC) for financial support (grant BB/M017702/1). This is a contribution from the Manchester Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM). D.B.K. thanks Dr Steve O{\textquoteright}Hagan for a statistical analysis. S.G.O. thanks both the BBSRC and the UK Technology Strategy Board (grants BB/C5051140/2 and BB/L004437/1: {\textquoteleft}13TSB_SynBio{\textquoteright}), as well the European Commission (7th Framework Programme BIOLEDGE Contract 289126), for research funds. We apologise to the many readers whose work was not cited due to stringent limitations on the number of citations.",

year = "2015",

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doi = "10.1016/j.tibtech.2015.02.001",

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journal = "Trends in Biotechnology",

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AU - Kell, Douglas

AU - Swainston, Neil

AU - Pir, P

AU - Oliver, SG

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PY - 2015/3

Y1 - 2015/3

N2 - Because they mainly do not involve chemical changes, membrane transporters have been a Cinderella subject in the biotechnology of small molecule production, but this is a serious oversight. Influx transporters contribute significantly to the flux towards product, and efflux transporters ensure the accumulation of product in the much greater extracellular space of fermentors. Programmes for improving biotechnological processes might therefore give greater consideration to transporters than may have been commonplace. Strategies for identifying important transporters include expression profiling, genome-wide knockout studies, stress-based selection, and the use of inhibitors. In addition, modern methods of directed evolution and synthetic biology, especially those effecting changes in energy coupling, offer huge opportunities for increasing the flux towards extracellular product formation by transporter engineering.

AB - Because they mainly do not involve chemical changes, membrane transporters have been a Cinderella subject in the biotechnology of small molecule production, but this is a serious oversight. Influx transporters contribute significantly to the flux towards product, and efflux transporters ensure the accumulation of product in the much greater extracellular space of fermentors. Programmes for improving biotechnological processes might therefore give greater consideration to transporters than may have been commonplace. Strategies for identifying important transporters include expression profiling, genome-wide knockout studies, stress-based selection, and the use of inhibitors. In addition, modern methods of directed evolution and synthetic biology, especially those effecting changes in energy coupling, offer huge opportunities for increasing the flux towards extracellular product formation by transporter engineering.

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JO - Trends in Biotechnology

JF - Trends in Biotechnology

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ER -

Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis

Abstract

Keywords

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Projects

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

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