Meta-analysis of Expression of Hepatic Organic Anion Transporting Polypeptide (OATP) Transporters in Cellular Systems Relative to Human Liver Tissue

Justine Badée, Brahim Achour, Amin Rostami-Hochaghan, Aleksandra Galetin

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Organic anion transporting polypeptide (OATP)1B1, OATP1B3 and OATP2B1 transporters play an important role in hepatic drug disposition. Recently, an increasing number of studies have reported proteomic expression data for OATP transporters. However, systematic analysis and understanding of the actual differences in OATP expression between liver tissue and commonly used cellular systems is lacking. In the current study, meta-analysis was performed to assess the protein expression of OATP transporters reported in hepatocytes relative to liver tissue and identify any potential correlations in transporter expression levels in the same individual. OATP1B1 was identified as the most abundant uptake transporter at 5.9 ± 8.3, 5.8 ± 3.3 and 4.2 ± 1.7 fmol/μg protein in liver tissue, sandwich-cultured human hepatocytes (SCHH) and cryopreserved suspended hepatocytes, respectively. The rank order in average expression in liver tissue and cellular systems was OATP1B1 > OATP1B3 ≈ OATP2B1. Abundance levels of the OATP transporters investigated were not significantly different between liver and cellular systems, with the exception of OATP2B1 expression in SCHH relative to liver tissue. Analysis of OATP1B1, OATP1B3 and OATP2B1 liver expression data in the same individuals (n = 86) identified weak (OATP1B1/OATP2B1) to moderate (OATP1B3/OATP2B1) significant correlations. A significant weak correlation was noted between OATP1B1 abundance and age of human donors, whereas expression of the OATPs investigated was independent of sex. Implications of the current analysis on the in vitro-in vivo extrapolation of transporter-mediated drug disposition using physiologically-based pharmacokinetic models are discussed.
    Original languageEnglish
    Pages (from-to)424-432
    Number of pages9
    JournalDrug Metabolism and Disposition
    Volume43
    Issue number4
    DOIs
    Publication statusPublished - Apr 2015

    Keywords

    • Hepatocytes, physiologically-based pharmacokinetic modeling/PBPK, proteomics, Transporter-mediated drug/metabolite disposition, Uptake transporters (OATP, OAT, OCT, PEPT, MCT, NTCP, ASBT, etc.)

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