Abstract
Chinese Hamster Ovary (CHO) cells are the main expression system for production of therapeutic recombinant (r-) proteins. The increased demand for these therapeutics has boosted the development of more robust production processes. Use of optimised feed/media has dramatically improved the performance of CHO cells in culture. However, such progress in biomass synthesis and r-protein production often come with an increased accumulation of lactate. In this study, we present a combined feeding strategy that uses galactose and lactate to replace glucose in CHO cell cultures. Replacement of glucose by galactose and lactate sustained cell growth and r-protein production in CHO cells. This strategy supported a better-balanced and more efficient metabolism, observed by an overall decreased consumption of carbon sources and amino acids, associated with an increased ATP production per C-mol consumed. Our results provide new insights of CHO cell metabolism in glucose-free media based on galactose and lactate.
Original language | English |
---|---|
Pages (from-to) | 201-211 |
Number of pages | 11 |
Journal | Chemical Engineering Science |
Volume | 205 |
Early online date | 2 May 2019 |
DOIs | |
Publication status | Published - 21 Sept 2019 |
Keywords
- Biochemical engineering
- Biopharmaceuticals
- Bioprocessing
- Lactate
- Metabolic flux analysis
- Metabolism modelling
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology