Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

Warwick B. Dunn; David Broadhurst; Marie Brown; Philip N. Baker; Christopher W G Redman; Louise C. Kenny; Douglas B. Kell

doi:10.1016/j.jchromb.2008.03.021

Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

Warwick B. Dunn, David Broadhurst, Marie Brown, Philip N. Baker, Christopher W G Redman, Louise C. Kenny, Douglas B. Kell

The University of Manchester

Research output: Contribution to journal › Article › peer-review

Abstract

Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 μm liquid chromatography (UPLC ACQUITY®) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 μmol L-1. Reproducibility of retention time (RSD <3%) and chromatographic peak area (RSD <15%) and a high mass accuracy (

Original language	English
Pages (from-to)	288-298
Number of pages	10
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	871
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2008.03.021
Publication status	Published - 15 Aug 2008

Keywords

Metabolic profiling
Orbitrap
Pre-eclampsia
UPLC
UPLC-MS
XCMS

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10.1016/j.jchromb.2008.03.021

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18420470

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Dunn, W. B., Broadhurst, D., Brown, M., Baker, P. N., Redman, C. W. G., Kenny, L. C., & Kell, D. B. (2008). Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 871(2), 288-298. https://doi.org/10.1016/j.jchromb.2008.03.021

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title = "Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system",

abstract = "Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 μm liquid chromatography (UPLC ACQUITY{\textregistered}) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 μmol L-1. Reproducibility of retention time (RSD <3%) and chromatographic peak area (RSD <15%) and a high mass accuracy (",

keywords = "Metabolic profiling, Orbitrap, Pre-eclampsia, UPLC, UPLC-MS, XCMS",

author = "Dunn, {Warwick B.} and David Broadhurst and Marie Brown and Baker, {Philip N.} and Redman, {Christopher W G} and Kenny, {Louise C.} and Kell, {Douglas B.}",

year = "2008",

month = aug,

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doi = "10.1016/j.jchromb.2008.03.021",

language = "English",

volume = "871",

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journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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Dunn, WB, Broadhurst, D, Brown, M, Baker, PN, Redman, CWG, Kenny, LC & Kell, DB 2008, 'Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 871, no. 2, pp. 288-298. https://doi.org/10.1016/j.jchromb.2008.03.021

Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. / Dunn, Warwick B.; Broadhurst, David; Brown, Marie et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 871, No. 2, 15.08.2008, p. 288-298.

Research output: Contribution to journal › Article › peer-review

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T1 - Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

AU - Dunn, Warwick B.

AU - Broadhurst, David

AU - Brown, Marie

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AU - Redman, Christopher W G

AU - Kenny, Louise C.

AU - Kell, Douglas B.

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KW - Orbitrap

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KW - UPLC

KW - UPLC-MS

KW - XCMS

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Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

Abstract

Keywords

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