Metabolomic characterization of the salt stress response in streptomyces coelicolor

Stefan Kol, M. Elena Merlo, Richard A. Scheltema, Marcel De Vries, Roel J. Vonk, Niels A. Kikkert, Lubbert Dijkhuizen, Rainer Breitling, Eriko Takano

    Research output: Contribution to journalArticlepeer-review


    The humicolous actinomycete Streptomyces coelicolor routinely adapts to a wide variety of habitats and rapidly changing environments. Upon salt stress, the organism is also known to increase the levels of various compatible solutes. Here we report the results of the first high-resolution metabolomics time series analysis of various strains of S. coelicolor exposed to salt stress: the wild type, mutants with progressive knockouts of the ectoine biosynthesis pathway, and two stress regulator mutants (with disruptions of the sigB and osaB genes). Samples were taken from cultures at 0, 4, 8, and 24 h after salt stress treatment and analyzed by liquid chromatography-mass spectrometry with an LTQ Orbitrap XL mass spectrometer. The results suggest that a large fraction of amino acids is upregulated in response to the salt stress, as are proline/glycine-containing diand tripeptides. Additionally we found that 5'-methylthioadenosine, a known inhibitor of polyamine biosynthesis, is downregulated upon salt stress. Strikingly, no major differences between the wild-type cultures and the two stress regulator mutants were found, indicating a considerable robustness of the metabolomic response to salt stress, compared to the more volatile changes in transcript abundance reported earlier. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)2574-2581
    Number of pages7
    JournalApplied and environmental microbiology
    Issue number8
    Publication statusPublished - Apr 2010


    Dive into the research topics of 'Metabolomic characterization of the salt stress response in streptomyces coelicolor'. Together they form a unique fingerprint.

    Cite this