Metal binding to a zinc-finger peptide: A comparison between solution and the gas phase

Yana Berezovskaya; Craig T. Armstrong; Aimee L. Boyle; Massimiliano Porrini; Derek N. Woolfson; Perdita E. Barran

doi:Doi 10.1039/C0cc02445g

Metal binding to a zinc-finger peptide: A comparison between solution and the gas phase

Yana Berezovskaya, Craig T. Armstrong, Aimee L. Boyle, Massimiliano Porrini, Derek N. Woolfson, Perdita E. Barran

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Abstract

Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys 2His 2 zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations. © 2011 The Royal Society of Chemistry.

Original language	English
Pages (from-to)	412-414
Number of pages	2
Journal	Chemical Communications
Volume	47
Issue number	1
DOIs	https://doi.org/Doi 10.1039/C0cc02445g
Publication status	Published - 7 Jan 2011

Keywords

mass-spectrometry

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abstract = "Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys 2His 2 zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations. {\textcopyright} 2011 The Royal Society of Chemistry.",

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AU - Berezovskaya, Yana

AU - Armstrong, Craig T.

AU - Boyle, Aimee L.

AU - Porrini, Massimiliano

AU - Woolfson, Derek N.

AU - Barran, Perdita E.

N1 - 691GM Times Cited:9 Cited References Count:10

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N2 - Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys 2His 2 zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations. © 2011 The Royal Society of Chemistry.

AB - Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys 2His 2 zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations. © 2011 The Royal Society of Chemistry.

KW - mass-spectrometry

U2 - Doi 10.1039/C0cc02445g

DO - Doi 10.1039/C0cc02445g

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SP - 412

EP - 414

JO - Chemical Communications

JF - Chemical Communications

IS - 1

ER -

Metal binding to a zinc-finger peptide: A comparison between solution and the gas phase

Abstract

Keywords

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