Method for showing human spermatogenesis using Arachis hypogaea (AHA) lectin

Carolyn Jones; E.W. Benbow; W. Fiona Knox; R.W. Stoddart

doi:10.1136/jcp.41.9.1017

Method for showing human spermatogenesis using Arachis hypogaea (AHA) lectin

Carolyn Jones, E.W. Benbow, W. Fiona Knox, R.W. Stoddart

Research output: Contribution to journal › Article › peer-review

Abstract

Using biotinylated Arachis hypogaea agglutinin (AHA) or peanut lectin (PNA) and an avidin-peroxidase procedure, the various stages of spermatid development were visualised with great clarity; a light haematoxylin counterstain permitted the easy recognition ofspermatogenic cells in the same section. This method is particularly useful in the interpretation ofpoorly fixed material and may be helpful in studies of cyclical maturation of spermatozoa, irrespective of whether the material is obtained at biopsy or necropsy.

Original language	Undefined
Pages (from-to)	1017-1018
Number of pages	2
Journal	Journal of Clinical Pathology
Volume	41
Issue number	9
DOIs	https://doi.org/10.1136/jcp.41.9.1017
Publication status	Published - 1988

Keywords

agglutinin
arachis oil
lectin, histochemistry
human
human cell
male
methodology
peanut lectin
priority journal
spermatogenesis, Acrosome
Arachis hypogaea
Histological Techniques
Human
Lectins
Male
Peanut Agglutinin
Spermatogenesis
Testis

Access to Document

10.1136/jcp.41.9.1017

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023713297&partnerID=40&md5=c5afa959e5875fb92ad039b6e07a39f6

Cite this

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title = "Method for showing human spermatogenesis using Arachis hypogaea (AHA) lectin",

abstract = "Using biotinylated Arachis hypogaea agglutinin (AHA) or peanut lectin (PNA) and an avidin-peroxidase procedure, the various stages of spermatid development were visualised with great clarity; a light haematoxylin counterstain permitted the easy recognition ofspermatogenic cells in the same section. This method is particularly useful in the interpretation ofpoorly fixed material and may be helpful in studies of cyclical maturation of spermatozoa, irrespective of whether the material is obtained at biopsy or necropsy.",

keywords = "agglutinin, arachis oil, lectin, histochemistry, human, human cell, male, methodology, peanut lectin, priority journal, spermatogenesis, Acrosome, Arachis hypogaea, Histological Techniques, Human, Lectins, Male, Peanut Agglutinin, Spermatogenesis, Testis",

author = "Carolyn Jones and E.W. Benbow and {Fiona Knox}, W. and R.W. Stoddart",

note = "cited By 4",

year = "1988",

doi = "10.1136/jcp.41.9.1017",

language = "Undefined",

volume = "41",

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TY - JOUR

T1 - Method for showing human spermatogenesis using Arachis hypogaea (AHA) lectin

AU - Jones, Carolyn

AU - Benbow, E.W.

AU - Fiona Knox, W.

AU - Stoddart, R.W.

N1 - cited By 4

PY - 1988

Y1 - 1988

N2 - Using biotinylated Arachis hypogaea agglutinin (AHA) or peanut lectin (PNA) and an avidin-peroxidase procedure, the various stages of spermatid development were visualised with great clarity; a light haematoxylin counterstain permitted the easy recognition ofspermatogenic cells in the same section. This method is particularly useful in the interpretation ofpoorly fixed material and may be helpful in studies of cyclical maturation of spermatozoa, irrespective of whether the material is obtained at biopsy or necropsy.

AB - Using biotinylated Arachis hypogaea agglutinin (AHA) or peanut lectin (PNA) and an avidin-peroxidase procedure, the various stages of spermatid development were visualised with great clarity; a light haematoxylin counterstain permitted the easy recognition ofspermatogenic cells in the same section. This method is particularly useful in the interpretation ofpoorly fixed material and may be helpful in studies of cyclical maturation of spermatozoa, irrespective of whether the material is obtained at biopsy or necropsy.

KW - agglutinin

KW - arachis oil

KW - lectin, histochemistry

KW - human

KW - human cell

KW - male

KW - methodology

KW - peanut lectin

KW - priority journal

KW - spermatogenesis, Acrosome

KW - Arachis hypogaea

KW - Histological Techniques

KW - Human

KW - Lectins

KW - Male

KW - Peanut Agglutinin

KW - Spermatogenesis

KW - Testis

U2 - 10.1136/jcp.41.9.1017

DO - 10.1136/jcp.41.9.1017

M3 - Article

SN - 1472-4146

VL - 41

SP - 1017

EP - 1018

JO - Journal of Clinical Pathology

JF - Journal of Clinical Pathology

IS - 9

ER -