Abstract
Posttranslational modifications are crucial in determining the functions of proteins in the cell. Modification of the NLRP3 inflammasome by the ubiquitin system has recently emerged as a new level of regulation of the inflammasome complex. Here, we describe a method to detect polyubiquitination of NRLP3 using two different approaches: (1) detection with an ubiquin antibody or (2) using TUBE (Tandem Ubiquitin Binding entities). This approach can be used to detect ubiquitination of other NLR or other components of the inflammasome.
| Original language | English |
|---|---|
| Title of host publication | NLR proteins : methods and protocols |
| Editors | Francesco Di Virgilio, Pablo Pelegrin |
| Publisher | Springer Nature |
| Pages | 223-229 |
| Volume | 1417 |
| ISBN (Electronic) | 978-1-4939-3566-6 |
| ISBN (Print) | 978-1-4939-3564-2 |
| Publication status | Published - 2016 |