Method to Measure Ubiquitination of NLRs.

Pablo Palazón, Gloria Lopez-Castejon

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Posttranslational modifications are crucial in determining the functions of proteins in the cell. Modification of the NLRP3 inflammasome by the ubiquitin system has recently emerged as a new level of regulation of the inflammasome complex. Here, we describe a method to detect polyubiquitination of NRLP3 using two different approaches: (1) detection with an ubiquin antibody or (2) using TUBE (Tandem Ubiquitin Binding entities). This approach can be used to detect ubiquitination of other NLR or other components of the inflammasome.
Original languageEnglish
Title of host publicationNLR proteins : methods and protocols
EditorsFrancesco Di Virgilio, Pablo Pelegrin
PublisherSpringer Nature
Pages223-229
Volume1417
ISBN (Electronic)978-1-4939-3566-6
ISBN (Print)978-1-4939-3564-2
Publication statusPublished - 2016

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