@inbook{ec60bcc1f0954d51acffebb464ce2a47,
title = "Method to Measure Ubiquitination of NLRs",
abstract = "Posttranslational modifications are crucial in determining the functions of proteins in the cell. Modification of the NLRP3 inflammasome by the ubiquitin system has recently emerged as a new level of regulation of the inflammasome complex. Here we describe a method to detect poly-ubiquitination of NRLP3 using two different approaches: (i) detection with a ubiquitin antibody or (ii) using TUBEs (Tandem Ubiquitin Binding entities). This approach can be used to detect ubiquitination of other NLRs or other proteins.",
keywords = "Inflammasomes/metabolism, NLR Family, Pyrin Domain-Containing 3 Protein/metabolism, Ubiquitination, Ubiquitin/metabolism, Protein Processing, Post-Translational",
author = "Worboys, {Jonathan D} and Pablo Palaz{\'o}n-Riquelme and Gloria L{\'o}pez-Castej{\'o}n",
note = "{\textcopyright} 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
month = aug,
day = "15",
doi = "10.1007/978-1-0716-3350-2_7",
language = "English",
isbn = "978-1-0716-3349-6",
volume = "2696",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer Nature",
pages = "105--114",
editor = "Pablo Pelegr{\'i}n and {Di Virgilio}, Francesco",
booktitle = "NLR Proteins",
address = "United States",
edition = "2",
}