Methods to study plant programmed cell death

Joanna Kacprzyk, Adrian N. Dauphinee, Patrick Gallois, Arunika HLAN Gunawardena, Paul F. McCabe

    Research output: Chapter in Book/Conference proceedingChapterpeer-review

    Abstract

    Programmed cell death (PCD) is a critical component of plant development, defense against invading pathogens, and response to environmental stresses. In this chapter, we provide detailed technical methods for studying PCD associated with plant development or induced by abiotic stress. A root hair assay or electrolyte leakage assay are excellent techniques for the quantitative determination of PCD and/or cellular injury induced in response to abiotic stress, whereas the lace plant provides a unique model that facilitates the study of genetically regulated PCD during leaf development.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press, Inc
    Pages145-160
    Number of pages16
    Volume1419
    DOIs
    Publication statusPublished - 1 Apr 2016

    Publication series

    NameMethods in Molecular Biology
    Volume1419
    ISSN (Print)10643745

    Keywords

    • Abiotic stress
    • Developmental programmed cell death
    • Electrolyte leakage
    • Leaf morphogenesis
    • Programmed cell death
    • Root hair assay

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