Abstract
A microfluidic device and method for the monitoring, manipulation and/or detection of chem. and biol. samples wherein an aq. reaction mixt. comprising a sample is introduced into a vol. of a carrier fluid immiscible with the aq. reaction mixt. to form a droplet of the aq. mixt. supported by the carrier fluid. The sensitivity of the method is regulated by controlling the vol. of aq. mixt. used to form the droplet and is particularly suitable for detecting analytes from a minor population of cells within mixed populations of cells. [on SciFinder (R)]
| Original language | English |
|---|---|
| Patent number | 2004-GB2850 2005002730 |
| Publication status | Published - 2005 |
Keywords
- Drops (microdroplets
- microfluidic method and device)
- Lab-on-a-chip (microfluidic device
- Cell
- Fluorescence resonance energy transfer
- Nucleic acid hybridization
- PCR
- Sample preparation (microfluidic method and device)
- DNA Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (microfluidic method and device)
- Primers Role: ARU (Analytical role, unclassified), ANST (Analytical study) (microfluidic method and device)
- microfluidic device
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