Microscale solution isoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome

Niroshini Nirmalan; Fiona Flett; Tom Skinner; John E. Hyde; P. F G Sims

doi:10.1021/pr070278r

Microscale solution isoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome

Niroshini Nirmalan, Fiona Flett, Tom Skinner, John E. Hyde, P. F G Sims

Research output: Contribution to journal › Article › peer-review

Abstract

The high hemeozoin (beta-hemeatin) content of Plasmodium falciparum lysates imposes severe limitations on the analysis of the malarial proteome, in particular compromising the loading capacities of two-dimensional gels. Here we report on the adaptation of a recently developed solution-phase isoelectric focusing-based fractionation technique as a prefractionation strategy for efficient containment of hemeoglobin-derived products and complexity reduction, to facilitate the high-resolution gel-based quantitative analysis of plasmodial lysates. © 2007 American Chemical Society.

Original language	English
Pages (from-to)	3780-3787
Number of pages	7
Journal	Journal of Proteome Research
Volume	6
Issue number	9
DOIs	https://doi.org/10.1021/pr070278r
Publication status	Published - Sept 2007

Keywords

Malaria
Proteomics
Quantitation
Serine hydroxymethyltransferase
Two-dimensional gel electrophoresis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/pr070278r

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@article{ec01ecbcb8f442c8a1b8ebc8677b0c9d,

title = "Microscale solution isoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome",

abstract = "The high hemeozoin (beta-hemeatin) content of Plasmodium falciparum lysates imposes severe limitations on the analysis of the malarial proteome, in particular compromising the loading capacities of two-dimensional gels. Here we report on the adaptation of a recently developed solution-phase isoelectric focusing-based fractionation technique as a prefractionation strategy for efficient containment of hemeoglobin-derived products and complexity reduction, to facilitate the high-resolution gel-based quantitative analysis of plasmodial lysates. {\textcopyright} 2007 American Chemical Society.",

keywords = "Malaria, Proteomics, Quantitation, Serine hydroxymethyltransferase, Two-dimensional gel electrophoresis",

author = "Niroshini Nirmalan and Fiona Flett and Tom Skinner and Hyde, {John E.} and Sims, {P. F G}",

year = "2007",

month = sep,

doi = "10.1021/pr070278r",

language = "English",

volume = "6",

pages = "3780--3787",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

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}

Microscale solution isoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome. / Nirmalan, Niroshini; Flett, Fiona; Skinner, Tom et al.
In: Journal of Proteome Research, Vol. 6, No. 9, 09.2007, p. 3780-3787.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Microscale solution isoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome

AU - Nirmalan, Niroshini

AU - Flett, Fiona

AU - Skinner, Tom

AU - Hyde, John E.

AU - Sims, P. F G

PY - 2007/9

Y1 - 2007/9

N2 - The high hemeozoin (beta-hemeatin) content of Plasmodium falciparum lysates imposes severe limitations on the analysis of the malarial proteome, in particular compromising the loading capacities of two-dimensional gels. Here we report on the adaptation of a recently developed solution-phase isoelectric focusing-based fractionation technique as a prefractionation strategy for efficient containment of hemeoglobin-derived products and complexity reduction, to facilitate the high-resolution gel-based quantitative analysis of plasmodial lysates. © 2007 American Chemical Society.

AB - The high hemeozoin (beta-hemeatin) content of Plasmodium falciparum lysates imposes severe limitations on the analysis of the malarial proteome, in particular compromising the loading capacities of two-dimensional gels. Here we report on the adaptation of a recently developed solution-phase isoelectric focusing-based fractionation technique as a prefractionation strategy for efficient containment of hemeoglobin-derived products and complexity reduction, to facilitate the high-resolution gel-based quantitative analysis of plasmodial lysates. © 2007 American Chemical Society.

KW - Malaria

KW - Proteomics

KW - Quantitation

KW - Serine hydroxymethyltransferase

KW - Two-dimensional gel electrophoresis

U2 - 10.1021/pr070278r

DO - 10.1021/pr070278r

M3 - Article

C2 - 17696383

SN - 1535-3893

VL - 6

SP - 3780

EP - 3787

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 9

ER -

Microscale solution isoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome

Abstract

Keywords

UN SDGs

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