Microscopic imaging of DNA repair foci in irradiated normal tissues

Nirmal Bhogal; Farid Jalali; Robert G. Bristow

doi:10.1080/09553000902785791

Microscopic imaging of DNA repair foci in irradiated normal tissues

Nirmal Bhogal, Farid Jalali, Robert G. Bristow

Division of Cancer Sciences (L5)

Research output: Contribution to journal › Review article › peer-review

Abstract

Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as γ-H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy invitro and invivo in measuring intranuclear foci following irradiation.

Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.

Original language	English
Pages (from-to)	732-746
Number of pages	15
Journal	International Journal of Radiation Biology
Volume	85
Issue number	9
DOIs	https://doi.org/10.1080/09553000902785791
Publication status	Published - Sept 2009

Keywords

γ-H2AX
Biomarker
Confocal microscopy
Deconvolution
DNA repair
Foci
Ionising radiation
Normal tissues

Research Beacons, Institutes and Platforms

Manchester Cancer Research Centre

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10.1080/09553000902785791

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title = "Microscopic imaging of DNA repair foci in irradiated normal tissues",

abstract = "Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as γ-H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy invitro and invivo in measuring intranuclear foci following irradiation. Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.",

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AU - Jalali, Farid

AU - Bristow, Robert G.

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N2 - Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as γ-H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy invitro and invivo in measuring intranuclear foci following irradiation. Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.

AB - Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as γ-H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy invitro and invivo in measuring intranuclear foci following irradiation. Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.

KW - γ-H2AX

KW - Biomarker

KW - Confocal microscopy

KW - Deconvolution

KW - DNA repair

KW - Foci

KW - Ionising radiation

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Microscopic imaging of DNA repair foci in irradiated normal tissues

Abstract

Keywords

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