Microsphere-coupled scanning laser confocal nanoscope for sub-diffraction-limited imaging at 25 nm lateral resolution in the visible spectrum

Yinzhou Yan; Lin Li; Chao Feng; Wei Guo; Seoungjun Lee; Minghui Hong

doi:10.1021/nn406201q

Microsphere-coupled scanning laser confocal nanoscope for sub-diffraction-limited imaging at 25 nm lateral resolution in the visible spectrum

Yinzhou Yan, Lin Li, Chao Feng, Wei Guo, Seoungjun Lee, Minghui Hong

Mechanical and Aerospace Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We report a direct optical super-resolution imaging approach with 25 nm (similar to lambda/17) lateral resolution under 488 nm wavelength illumination by combining fused silica and polystyrene microspheres with a conventional scanning laser confocal microscope (SLCM). The microsphere deposited on the target surface generates a nanoscale central lobe illuminating a sub-diffraction-limited cross-section located on the target surface. The SLCM confocal pinhole isolates the reflected light from the near-field subdiffractive cross-section and suppresses the noises from the side lobe and the far-field paraxial focal point. The structural detail of the subdiffractive cross-section is therefore captured, and the 2D target surface near the bottom of microspheres can be imaged by intensity-based point scanning.

Original language	English
Pages (from-to)	1809-1816
Number of pages	8
Journal	A C S Nano
Volume	8
Issue number	2
DOIs	https://doi.org/10.1021/nn406201q
Publication status	Published - Feb 2014

Keywords

super-resolution; imaging; microsphere; scanning laser confocal microscope; nanoscope; diffraction limit

Access to Document

10.1021/nn406201q

LPRC: Laser Processing Research Centre Impact
Allegre, O. (PI), Crosby, D. (PI), Domingos, M. (PI), Francis, J. (PI), Gillen, D. (PI), Guo, W. (PI), Li, L. (PI), Mativenga, P. (PI), Rogers, B. D. (PI), Whitehead, D. (PI), Wilson, D. (PI), Zhong, S. (PI), Mirhosseini, N. (Researcher), Ouyang, J. (Researcher), Huang, Y. (Researcher), Wei, C. (Researcher), Cheng, D. (PGR student), Suryo Pratomo, E. (PGR student), Zhang, Z. (PGR student), Li, Z. (PGR student), Mo, H. (PGR student), Zhu, M. (PGR student), Li, Q. (PGR student), Liu, W. (PGR student), Wang, L. (PGR student), Guo, H. (PGR student), Heinemann, R. (PI) & Liu, Z. (PI)
1/04/00 → …
Project: Research

Cite this

@article{ed51a65a549a478db8a48c7f7d4a4525,

title = "Microsphere-coupled scanning laser confocal nanoscope for sub-diffraction-limited imaging at 25 nm lateral resolution in the visible spectrum",

abstract = "We report a direct optical super-resolution imaging approach with 25 nm (similar to lambda/17) lateral resolution under 488 nm wavelength illumination by combining fused silica and polystyrene microspheres with a conventional scanning laser confocal microscope (SLCM). The microsphere deposited on the target surface generates a nanoscale central lobe illuminating a sub-diffraction-limited cross-section located on the target surface. The SLCM confocal pinhole isolates the reflected light from the near-field subdiffractive cross-section and suppresses the noises from the side lobe and the far-field paraxial focal point. The structural detail of the subdiffractive cross-section is therefore captured, and the 2D target surface near the bottom of microspheres can be imaged by intensity-based point scanning.",

keywords = "super-resolution; imaging; microsphere; scanning laser confocal microscope; nanoscope; diffraction limit",

author = "Yinzhou Yan and Lin Li and Chao Feng and Wei Guo and Seoungjun Lee and Minghui Hong",

year = "2014",

month = feb,

doi = "10.1021/nn406201q",

language = "English",

volume = "8",

pages = "1809--1816",

journal = "A C S Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - Microsphere-coupled scanning laser confocal nanoscope for sub-diffraction-limited imaging at 25 nm lateral resolution in the visible spectrum

AU - Yan, Yinzhou

AU - Li, Lin

AU - Feng, Chao

AU - Guo, Wei

AU - Lee, Seoungjun

AU - Hong, Minghui

PY - 2014/2

Y1 - 2014/2

N2 - We report a direct optical super-resolution imaging approach with 25 nm (similar to lambda/17) lateral resolution under 488 nm wavelength illumination by combining fused silica and polystyrene microspheres with a conventional scanning laser confocal microscope (SLCM). The microsphere deposited on the target surface generates a nanoscale central lobe illuminating a sub-diffraction-limited cross-section located on the target surface. The SLCM confocal pinhole isolates the reflected light from the near-field subdiffractive cross-section and suppresses the noises from the side lobe and the far-field paraxial focal point. The structural detail of the subdiffractive cross-section is therefore captured, and the 2D target surface near the bottom of microspheres can be imaged by intensity-based point scanning.

AB - We report a direct optical super-resolution imaging approach with 25 nm (similar to lambda/17) lateral resolution under 488 nm wavelength illumination by combining fused silica and polystyrene microspheres with a conventional scanning laser confocal microscope (SLCM). The microsphere deposited on the target surface generates a nanoscale central lobe illuminating a sub-diffraction-limited cross-section located on the target surface. The SLCM confocal pinhole isolates the reflected light from the near-field subdiffractive cross-section and suppresses the noises from the side lobe and the far-field paraxial focal point. The structural detail of the subdiffractive cross-section is therefore captured, and the 2D target surface near the bottom of microspheres can be imaged by intensity-based point scanning.

KW - super-resolution; imaging; microsphere; scanning laser confocal microscope; nanoscope; diffraction limit

U2 - 10.1021/nn406201q

DO - 10.1021/nn406201q

M3 - Article

SN - 1936-0851

VL - 8

SP - 1809

EP - 1816

JO - A C S Nano

JF - A C S Nano

IS - 2

ER -

Microsphere-coupled scanning laser confocal nanoscope for sub-diffraction-limited imaging at 25 nm lateral resolution in the visible spectrum

Abstract

Keywords

Access to Document

Fingerprint

Projects

LPRC: Laser Processing Research Centre Impact

Cite this