Miniature Neurotransmission Regulates Drosophila Synaptic Structural Maturation

BenJiwon Choi, WendyL Imlach, Wei Jiao, Verena Wolfram, Ying Wu, Mark Grbic, Carolina Cela, RichardA Baines, MichaelN Nitabach, Brian D. McCabe

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Miniature neurotransmission is the transsynaptic process where single synaptic vesicles spontaneously released from presynaptic neurons induce miniature postsynaptic potentials. Since their discovery over 60 years ago, miniature events have been found at every chemical synapse studied. However, the invivo necessity for these small-amplitude events has remained enigmatic. Here, we show that miniature neurotransmission is required for the normal structural maturation of Drosophila glutamatergic synapses in a developmental role that is not shared by evoked neurotransmission. Conversely, we find that increasing miniature events is sufficient to induce synaptic terminal growth. We show that miniature neurotransmission acts locally at terminals to regulate synapse maturation via a Trio guanine nucleotide exchange factor (GEF) and Rac1 GTPase molecular signaling pathway. Our results establish that miniature neurotransmission, a universal but often-overlooked feature of synapses, has unique and essential functions invivo. © 2014 The Authors.
    Original languageEnglish
    Pages (from-to)618-634
    Number of pages16
    JournalNeuron
    Volume82
    Issue number3
    DOIs
    Publication statusPublished - 7 May 2014

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