Miniaturised free flow isotachophoresis of bacteria using an injection moulded separation device

Jeff E. Prest; Sara J. Baldock; Peter R. Fielden; Nicholas J. Goddard; Royston Goodacre; Richard O'Connor; Bernard J. Treves Brown

doi:10.1016/j.jchromb.2012.06.040

Miniaturised free flow isotachophoresis of bacteria using an injection moulded separation device

Jeff E. Prest, Sara J. Baldock, Peter R. Fielden, Nicholas J. Goddard, Royston Goodacre, Richard O'Connor, Bernard J. Treves Brown

Research output: Contribution to journal › Article › peer-review

Abstract

A new design of miniaturised free flow electrophoresis device has been produced. The design contains a separation chamber that is 45. mm long by 31.7. mm wide with a depth of 50 μm and has nine inlet and nine outlet holes to allow for fraction collection. The devices were formed of polystyrene with carbon fibre loaded polystyrene drive electrodes and produced using injection moulding. This means that the devices are low cost and can potentially be mass produced. The devices were used for free flow isotachophoresis (FFITP), a technique that can be used for focussing and concentrating analytes contained within complex sample matrices. The operation of the devices was demonstrated by performing separations of dyes and bacterial samples. Analysis of the output from FFITP separations of samples containing the bacterium Erwinia herbicola, a biological pathogen, by cell culturing and counting showed that fractionation of the output was achieved. © 2012 Elsevier B.V.

Original language	English
Pages (from-to)	53-59
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	903
DOIs	https://doi.org/10.1016/j.jchromb.2012.06.040
Publication status	Published - 15 Aug 2012

Keywords

*Bacteriological Techniques: IS, instrumentation
Bacteriological Techniques: MT, methods
Coloring Agents: IP, isolation & purification
Equipment Design
*Erwinia: IP, isolation & purification
*Isotachophoresis: IS, instrumentation
Isotachophoresis: MT, methods
*Miniaturization: IS, instrumentation
0 (Coloring Agents)

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10.1016/j.jchromb.2012.06.040

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@article{ecba2c8002e248c6b8062e686047ab7b,

title = "Miniaturised free flow isotachophoresis of bacteria using an injection moulded separation device",

abstract = "A new design of miniaturised free flow electrophoresis device has been produced. The design contains a separation chamber that is 45. mm long by 31.7. mm wide with a depth of 50 μm and has nine inlet and nine outlet holes to allow for fraction collection. The devices were formed of polystyrene with carbon fibre loaded polystyrene drive electrodes and produced using injection moulding. This means that the devices are low cost and can potentially be mass produced. The devices were used for free flow isotachophoresis (FFITP), a technique that can be used for focussing and concentrating analytes contained within complex sample matrices. The operation of the devices was demonstrated by performing separations of dyes and bacterial samples. Analysis of the output from FFITP separations of samples containing the bacterium Erwinia herbicola, a biological pathogen, by cell culturing and counting showed that fractionation of the output was achieved. {\textcopyright} 2012 Elsevier B.V.",

keywords = "*Bacteriological Techniques: IS, instrumentation, Bacteriological Techniques: MT, methods, Coloring Agents: IP, isolation & purification, Equipment Design, *Erwinia: IP, isolation & purification, *Isotachophoresis: IS, instrumentation, Isotachophoresis: MT, methods, *Miniaturization: IS, instrumentation, 0 (Coloring Agents)",

author = "Prest, {Jeff E.} and Baldock, {Sara J.} and Fielden, {Peter R.} and Goddard, {Nicholas J.} and Royston Goodacre and Richard O'Connor and {Treves Brown}, {Bernard J.}",

note = "Netherlands FIELD Citation:",

year = "2012",

month = aug,

day = "15",

doi = "10.1016/j.jchromb.2012.06.040",

language = "English",

volume = "903",

pages = "53--59",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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T1 - Miniaturised free flow isotachophoresis of bacteria using an injection moulded separation device

AU - Prest, Jeff E.

AU - Baldock, Sara J.

AU - Fielden, Peter R.

AU - Goddard, Nicholas J.

AU - Goodacre, Royston

AU - O'Connor, Richard

AU - Treves Brown, Bernard J.

N1 - Netherlands FIELD Citation:

PY - 2012/8/15

Y1 - 2012/8/15

N2 - A new design of miniaturised free flow electrophoresis device has been produced. The design contains a separation chamber that is 45. mm long by 31.7. mm wide with a depth of 50 μm and has nine inlet and nine outlet holes to allow for fraction collection. The devices were formed of polystyrene with carbon fibre loaded polystyrene drive electrodes and produced using injection moulding. This means that the devices are low cost and can potentially be mass produced. The devices were used for free flow isotachophoresis (FFITP), a technique that can be used for focussing and concentrating analytes contained within complex sample matrices. The operation of the devices was demonstrated by performing separations of dyes and bacterial samples. Analysis of the output from FFITP separations of samples containing the bacterium Erwinia herbicola, a biological pathogen, by cell culturing and counting showed that fractionation of the output was achieved. © 2012 Elsevier B.V.

AB - A new design of miniaturised free flow electrophoresis device has been produced. The design contains a separation chamber that is 45. mm long by 31.7. mm wide with a depth of 50 μm and has nine inlet and nine outlet holes to allow for fraction collection. The devices were formed of polystyrene with carbon fibre loaded polystyrene drive electrodes and produced using injection moulding. This means that the devices are low cost and can potentially be mass produced. The devices were used for free flow isotachophoresis (FFITP), a technique that can be used for focussing and concentrating analytes contained within complex sample matrices. The operation of the devices was demonstrated by performing separations of dyes and bacterial samples. Analysis of the output from FFITP separations of samples containing the bacterium Erwinia herbicola, a biological pathogen, by cell culturing and counting showed that fractionation of the output was achieved. © 2012 Elsevier B.V.

KW - Bacteriological Techniques: IS, instrumentation

KW - Bacteriological Techniques: MT, methods

KW - Coloring Agents: IP, isolation & purification

KW - Equipment Design

KW - Erwinia: IP, isolation & purification

KW - Isotachophoresis: IS, instrumentation

KW - Isotachophoresis: MT, methods

KW - Miniaturization: IS, instrumentation

KW - 0 (Coloring Agents)

U2 - 10.1016/j.jchromb.2012.06.040

DO - 10.1016/j.jchromb.2012.06.040

M3 - Article

C2 - 22819202

SN - 1570-0232

VL - 903

SP - 53

EP - 59

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

ER -

Miniaturised free flow isotachophoresis of bacteria using an injection moulded separation device

Abstract

Keywords

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