Miniaturised isotachophoresis of DNA

Jeff E. Prest, Sara J. Baldock, Philip J R Day, Peter R. Fielden, Nicholas J. Goddard, Bernard J. Treves Brown

    Research output: Contribution to journalArticlepeer-review

    Abstract

    This paper presents the findings of a feasibility study investigating the behaviour of DNA under conditions of miniaturised isotachophoresis. An electrolyte system comprising a leading electrolyte of 5 mM perchloric acid at pH 6.0 and a terminating electrolyte of 10 mM gallic acid was devised and used to perform isotachophoresis of DNA containing samples on a miniaturised poly(methyl methacrylate) device. Under such conditions it was found that no separation of DNA fragments was observed with the substance migrating instead as a single isotachophoretic zone. Whilst such a result shows the method is unsuitable for analysis DNA it offers significant potential as a means of sample preparation for subsequent analysis using another method. This is because the single zone of DNA formed is preconcentrated to a constant concentration governed by the leading ion and is separated from all species with different effective electrophoretic mobilities. © 2007 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)154-159
    Number of pages5
    JournalJournal of Chromatography A
    Volume1156
    Issue number1-2
    DOIs
    Publication statusPublished - 13 Jul 2007

    Keywords

    • Chip
    • DNA
    • Isotachophoresis
    • Microdevice
    • Miniaturisation
    • Sample preparation

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