Minimized, CpG-depleted, and methylated DNA vectors: towards perfection in nonviral gene therapy

Oleg Tolmachov, Richard Harbottle, Brian Bigger, Charles Coutelle

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    Abstract

    This chapter contains sections titled:

    Introduction

    The Mammalian Immune System as a Barrier to Nonviral Gene Delivery

    Strategies to Minimize DNA Vectors

    Excision of a DNA Fragment Containing a Transgene Expression Cassette from Plasmid DNA

    Intramolecular Site-Specific Recombination Within a Bacterial Plasmid

    Synthesis of Minimized DNA Vectors by PCR

    Improvement of Minimized DNA Vector Yield and Purity

    Depletion of CpG Dinucleotides in the Bacterial Vector Backbone

    Methylation of CpG Dinucleotides in Plasmid DNA

    Towards an Ideal Nonviral Vector

    Conclusion

    References
    Original languageEnglish
    Title of host publicationDNA‐pharmaceuticals
    Subtitle of host publicationFormulation and delivery in gene therapy, DNA vaccination and immunotherapy
    EditorsMartin Schleef
    Place of PublicationWeinheim, Germany
    PublisherJohn Wiley & Sons Ltd
    Chapter4
    Pages43-54
    Number of pages12
    ISBN (Electronic)9783527607532
    ISBN (Print)9783527311873
    DOIs
    Publication statusPublished - 2005

    Keywords

    • DNA pharmaceuticals
    • nonviral gene therapy
    • minimized DNA vectors
    • CpG-depleted DNA vectors
    • methylated DNA vectors
    • mammalian immune system barrier to nonviral gene delivery
    • ideal nonviral vector

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