Minimizing tip-sample forces in jumping mode atomic force microscopy in liquid

A. Ortega-Esteban, I. Horcas, M. Hernando-Perez, P. Ares, A. J. Perez-Berna, C. San Martin, J. L. Carrascosa, P. J. de Pablo, J. Gomez-Herrero

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Control and minimization of tip–sample interaction forces are imperative tasks to maximize the performance of atomic force microscopy. In particular, when imaging soft biological matter in liquids, the cantilever dragging force prevents identification of the tip–sample mechanical contact, resulting in deleterious interaction with the specimen. In this work we present an improved jumping mode procedure that allows detecting the tip–sample contact with high accuracy, thus minimizing the scanning forces (∼100 pN) during the approach cycles. To illustrate this method we report images of human adenovirus and T7 bacteriophage particles which are prone to uncontrolled modifications when using conventional jumping mode.
    Original languageEnglish
    Pages (from-to)56-61
    Number of pages6
    JournalUltramicroscopy
    Volume114
    DOIs
    Publication statusPublished - Mar 2012

    Keywords

    • Atomic force microscopy
    • Scanning in liquids
    • Virus

    Fingerprint

    Dive into the research topics of 'Minimizing tip-sample forces in jumping mode atomic force microscopy in liquid'. Together they form a unique fingerprint.

    Cite this