Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase

C Tournier; A J Whitmarsh; J Cavanagh; T Barrett; R J Davis

Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase

C Tournier, A J Whitmarsh, J Cavanagh, T Barrett, R J Davis

Research output: Contribution to journal › Article › peer-review

Abstract

The c-Jun NH2-terminal kinase (JNK) group of mitogen-activated protein (MAP) kinases is activated by phosphorylation on Thr and Tyr. Here we report the molecular cloning of a new member of the mammalian MAP kinase kinase group (MKK7) that functions as an activator of JNK. In vitro protein kinase assays demonstrate that MKK7 phosphorylates and activates JNK, but not the p38 or extracellular signal-regulated kinase groups of MAP kinase. Expression of MKK7 in cultured cells causes activation of the JNK signal transduction pathway. MKK7 is therefore established to be a novel component of the JNK signal transduction pathway.

Original language	English
Pages (from-to)	7337-42
Number of pages	6
Journal	Proceedings of the National Academy of Sciences
Volume	94
Issue number	14
Publication status	Published - 8 Jul 1997

Keywords

Amino Acid Sequence
Animals
CHO Cells
Cloning, Molecular
Cricetinae
Gene Expression Regulation, Enzymologic
Humans
JNK Mitogen-Activated Protein Kinases
MAP Kinase Kinase 4
MAP Kinase Kinase 7
Mitogen-Activated Protein Kinase Kinases
Molecular Sequence Data
Protein Kinases
Sequence Alignment

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title = "Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase",

abstract = "The c-Jun NH2-terminal kinase (JNK) group of mitogen-activated protein (MAP) kinases is activated by phosphorylation on Thr and Tyr. Here we report the molecular cloning of a new member of the mammalian MAP kinase kinase group (MKK7) that functions as an activator of JNK. In vitro protein kinase assays demonstrate that MKK7 phosphorylates and activates JNK, but not the p38 or extracellular signal-regulated kinase groups of MAP kinase. Expression of MKK7 in cultured cells causes activation of the JNK signal transduction pathway. MKK7 is therefore established to be a novel component of the JNK signal transduction pathway.",

keywords = "Amino Acid Sequence, Animals, CHO Cells, Cloning, Molecular, Cricetinae, Gene Expression Regulation, Enzymologic, Humans, JNK Mitogen-Activated Protein Kinases, MAP Kinase Kinase 4, MAP Kinase Kinase 7, Mitogen-Activated Protein Kinase Kinases, Molecular Sequence Data, Protein Kinases, Sequence Alignment",

author = "C Tournier and Whitmarsh, {A J} and J Cavanagh and T Barrett and Davis, {R J}",

year = "1997",

month = jul,

day = "8",

language = "English",

volume = "94",

pages = "7337--42",

journal = "Proceedings of the National Academy of Sciences",

issn = "0027-8424",

publisher = "National Academy of Sciences",

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TY - JOUR

T1 - Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase

AU - Tournier, C

AU - Whitmarsh, A J

AU - Cavanagh, J

AU - Barrett, T

AU - Davis, R J

PY - 1997/7/8

Y1 - 1997/7/8

N2 - The c-Jun NH2-terminal kinase (JNK) group of mitogen-activated protein (MAP) kinases is activated by phosphorylation on Thr and Tyr. Here we report the molecular cloning of a new member of the mammalian MAP kinase kinase group (MKK7) that functions as an activator of JNK. In vitro protein kinase assays demonstrate that MKK7 phosphorylates and activates JNK, but not the p38 or extracellular signal-regulated kinase groups of MAP kinase. Expression of MKK7 in cultured cells causes activation of the JNK signal transduction pathway. MKK7 is therefore established to be a novel component of the JNK signal transduction pathway.

AB - The c-Jun NH2-terminal kinase (JNK) group of mitogen-activated protein (MAP) kinases is activated by phosphorylation on Thr and Tyr. Here we report the molecular cloning of a new member of the mammalian MAP kinase kinase group (MKK7) that functions as an activator of JNK. In vitro protein kinase assays demonstrate that MKK7 phosphorylates and activates JNK, but not the p38 or extracellular signal-regulated kinase groups of MAP kinase. Expression of MKK7 in cultured cells causes activation of the JNK signal transduction pathway. MKK7 is therefore established to be a novel component of the JNK signal transduction pathway.

KW - Amino Acid Sequence

KW - Animals

KW - CHO Cells

KW - Cloning, Molecular

KW - Cricetinae

KW - Gene Expression Regulation, Enzymologic

KW - Humans

KW - JNK Mitogen-Activated Protein Kinases

KW - MAP Kinase Kinase 4

KW - MAP Kinase Kinase 7

KW - Mitogen-Activated Protein Kinase Kinases

KW - Molecular Sequence Data

KW - Protein Kinases

KW - Sequence Alignment

M3 - Article

C2 - 9207092

SN - 0027-8424

VL - 94

SP - 7337

EP - 7342

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

IS - 14

ER -

Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase

Abstract

Keywords

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