Modification of the stability of phospholipase A2 by charge engineering

Richard W. Pickersgill; Ian G. Sumner; Margaret E. Collins; Jim Warwicker; Barry Perry; K. Mahalingeshwara Bhat; Peter W. Goodenough

doi:10.1016/0014-5793(91)80397-L

Modification of the stability of phospholipase A2 by charge engineering

Richard W. Pickersgill, Ian G. Sumner, Margaret E. Collins, Jim Warwicker, Barry Perry, K. Mahalingeshwara Bhat, Peter W. Goodenough

Research output: Contribution to journal › Article › peer-review

Abstract

Electrostatic interactions play an important role in stabilizing the folded conformation of globular proteins. Here we predict the change in stability of charge engineered mutants, construct these mutants and compare the predicted change in stability with that observed. The change in stability was correctly predicted for two of the three mutants and the factors responsible for the discrepancy between observation and prediction for the third mutant are discussed. © 1991.

Original language	English
Pages (from-to)	219-222
Number of pages	3
Journal	FEBS Letters
Volume	281
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80397-L
Publication status	Published - 9 Apr 1991

Keywords

Charge engineering
Protein engineering of phospholipase A2
Protein stability

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10.1016/0014-5793(91)80397-L

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title = "Modification of the stability of phospholipase A2 by charge engineering",

abstract = "Electrostatic interactions play an important role in stabilizing the folded conformation of globular proteins. Here we predict the change in stability of charge engineered mutants, construct these mutants and compare the predicted change in stability with that observed. The change in stability was correctly predicted for two of the three mutants and the factors responsible for the discrepancy between observation and prediction for the third mutant are discussed. {\textcopyright} 1991.",

keywords = "Charge engineering, Protein engineering of phospholipase A2, Protein stability",

author = "Pickersgill, {Richard W.} and Sumner, {Ian G.} and Collins, {Margaret E.} and Jim Warwicker and Barry Perry and Bhat, {K. Mahalingeshwara} and Goodenough, {Peter W.}",

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AU - Pickersgill, Richard W.

AU - Sumner, Ian G.

AU - Collins, Margaret E.

AU - Warwicker, Jim

AU - Perry, Barry

AU - Bhat, K. Mahalingeshwara

AU - Goodenough, Peter W.

PY - 1991/4/9

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N2 - Electrostatic interactions play an important role in stabilizing the folded conformation of globular proteins. Here we predict the change in stability of charge engineered mutants, construct these mutants and compare the predicted change in stability with that observed. The change in stability was correctly predicted for two of the three mutants and the factors responsible for the discrepancy between observation and prediction for the third mutant are discussed. © 1991.

AB - Electrostatic interactions play an important role in stabilizing the folded conformation of globular proteins. Here we predict the change in stability of charge engineered mutants, construct these mutants and compare the predicted change in stability with that observed. The change in stability was correctly predicted for two of the three mutants and the factors responsible for the discrepancy between observation and prediction for the third mutant are discussed. © 1991.

KW - Charge engineering

KW - Protein engineering of phospholipase A2

KW - Protein stability

U2 - 10.1016/0014-5793(91)80397-L

DO - 10.1016/0014-5793(91)80397-L

M3 - Article

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VL - 281

SP - 219

EP - 222

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Modification of the stability of phospholipase A2 by charge engineering

Abstract

Keywords

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