Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines.

Xin Wang

Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines.

Xin Wang^* (Corresponding)

^*Corresponding author for this work

Division of Cardiovascular Sciences (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.

Original language	English
Article number	Stem Cell Res . 2021 Apr:52:102238. doi: 10.1016/j.scr.2021.102238. Epub 2021 Feb 11.
Journal	Stem Cell Research
Publication status	Published - 11 Feb 2021

Cite this

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title = "Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines.",

abstract = "Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.",

author = "Xin Wang",

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journal = "Stem Cell Research",

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AB - Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.

M3 - Article

SN - 1873-5061

JO - Stem Cell Research

JF - Stem Cell Research

M1 - Stem Cell Res . 2021 Apr:52:102238. doi: 10.1016/j.scr.2021.102238. Epub 2021 Feb 11.

ER -

Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines.

Abstract

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