Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins

Cyrill Bussy; René Verhoef; Ash Haeger; Marco Morra; Jean Luc Duval; Pascale Vigneron; Anne Bensoussan; Elodie Velzenberger; Giovanna Cascardo; Clara Cassinelli; Henk Schols; J. Paul Knox; Marie Danielle Nagel

doi:10.1002/jbm.a.31729

Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins

Cyrill Bussy, René Verhoef, Ash Haeger, Marco Morra, Jean Luc Duval, Pascale Vigneron, Anne Bensoussan, Elodie Velzenberger, Giovanna Cascardo, Clara Cassinelli, Henk Schols, J. Paul Knox, Marie Danielle Nagel

Research output: Contribution to journal › Article › peer-review

Abstract

Previous work has reported the results of a multidisciplinary effort producing a proof-of-concept on the use of pectic polysaccharides in the surface modification of medical devices. This study was designed to learn more about the capability of engineered rhamnogalacturonan-I (RG-I) fractions of apple pectin to control bone cell and macrophage behavior. Thermanox® or polystyrene Petri dishes were surface modified with two different modified hairy regions (MHRs) obtained by different enzymatic liquefaction processes of apples differing in relative amounts and lengths of their neutral side chains: (long-haired) MHR-α and (short-haired) MHR-B. Bone expiants from 14-day-old chick embryos were cultured for 14 days on both pectic substrata. MHR-B promoted cell migration and differentiation, MHR-α did not. On MHR-α, J774.2 macrophages grew well, their percentage in G1 phase was decreased and in S phase increased, and they did not secrete either proinflammatory-cytokines or nitrites. Contrasting results were gained from macrophages on MHR-B, except for nitrite secretion. Thus, we conclude that coatings from tailored pectins show different biological activities in vitro and are potential innovative candidates for improving the biocompatibility of medical devices in various applications. © 2007 Wiley Periodicals.

Original language	English
Pages (from-to)	597-606
Number of pages	9
Journal	Journal of Biomedical Materials Research - Part A
Volume	86
Issue number	3
DOIs	https://doi.org/10.1002/jbm.a.31729
Publication status	Published - 1 Sept 2008

Keywords

Biocompatibility
Biomaterials
Biomimetic surfaces
Cell-surface interaction
Rhamnogalacturonan-I

Access to Document

10.1002/jbm.a.31729

Cite this

Bussy, C., Verhoef, R., Haeger, A., Morra, M., Duval, J. L., Vigneron, P., Bensoussan, A., Velzenberger, E., Cascardo, G., Cassinelli, C., Schols, H., Knox, J. P., & Nagel, M. D. (2008). Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins. Journal of Biomedical Materials Research - Part A, 86(3), 597-606. https://doi.org/10.1002/jbm.a.31729

@article{371fec6c0e7f4d4a8845addb8d81a1ea,

title = "Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins",

abstract = "Previous work has reported the results of a multidisciplinary effort producing a proof-of-concept on the use of pectic polysaccharides in the surface modification of medical devices. This study was designed to learn more about the capability of engineered rhamnogalacturonan-I (RG-I) fractions of apple pectin to control bone cell and macrophage behavior. Thermanox{\textregistered} or polystyrene Petri dishes were surface modified with two different modified hairy regions (MHRs) obtained by different enzymatic liquefaction processes of apples differing in relative amounts and lengths of their neutral side chains: (long-haired) MHR-α and (short-haired) MHR-B. Bone expiants from 14-day-old chick embryos were cultured for 14 days on both pectic substrata. MHR-B promoted cell migration and differentiation, MHR-α did not. On MHR-α, J774.2 macrophages grew well, their percentage in G1 phase was decreased and in S phase increased, and they did not secrete either proinflammatory-cytokines or nitrites. Contrasting results were gained from macrophages on MHR-B, except for nitrite secretion. Thus, we conclude that coatings from tailored pectins show different biological activities in vitro and are potential innovative candidates for improving the biocompatibility of medical devices in various applications. {\textcopyright} 2007 Wiley Periodicals.",

keywords = "Biocompatibility, Biomaterials, Biomimetic surfaces, Cell-surface interaction, Rhamnogalacturonan-I",

author = "Cyrill Bussy and Ren{\'e} Verhoef and Ash Haeger and Marco Morra and Duval, {Jean Luc} and Pascale Vigneron and Anne Bensoussan and Elodie Velzenberger and Giovanna Cascardo and Clara Cassinelli and Henk Schols and Knox, {J. Paul} and Nagel, {Marie Danielle}",

note = "334RD Times Cited:17 Cited References Count:59",

year = "2008",

month = sep,

day = "1",

doi = "10.1002/jbm.a.31729",

language = "English",

volume = "86",

pages = "597--606",

journal = "Journal of Biomedical Materials Research - Part A",

issn = "1549-3296",

publisher = "John Wiley & Sons Ltd",

number = "3",

}

Bussy, C, Verhoef, R, Haeger, A, Morra, M, Duval, JL, Vigneron, P, Bensoussan, A, Velzenberger, E, Cascardo, G, Cassinelli, C, Schols, H, Knox, JP & Nagel, MD 2008, 'Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins', Journal of Biomedical Materials Research - Part A, vol. 86, no. 3, pp. 597-606. https://doi.org/10.1002/jbm.a.31729

TY - JOUR

T1 - Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins

AU - Bussy, Cyrill

AU - Verhoef, René

AU - Haeger, Ash

AU - Morra, Marco

AU - Duval, Jean Luc

AU - Vigneron, Pascale

AU - Bensoussan, Anne

AU - Velzenberger, Elodie

AU - Cascardo, Giovanna

AU - Cassinelli, Clara

AU - Schols, Henk

AU - Knox, J. Paul

AU - Nagel, Marie Danielle

N1 - 334RD Times Cited:17 Cited References Count:59

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Previous work has reported the results of a multidisciplinary effort producing a proof-of-concept on the use of pectic polysaccharides in the surface modification of medical devices. This study was designed to learn more about the capability of engineered rhamnogalacturonan-I (RG-I) fractions of apple pectin to control bone cell and macrophage behavior. Thermanox® or polystyrene Petri dishes were surface modified with two different modified hairy regions (MHRs) obtained by different enzymatic liquefaction processes of apples differing in relative amounts and lengths of their neutral side chains: (long-haired) MHR-α and (short-haired) MHR-B. Bone expiants from 14-day-old chick embryos were cultured for 14 days on both pectic substrata. MHR-B promoted cell migration and differentiation, MHR-α did not. On MHR-α, J774.2 macrophages grew well, their percentage in G1 phase was decreased and in S phase increased, and they did not secrete either proinflammatory-cytokines or nitrites. Contrasting results were gained from macrophages on MHR-B, except for nitrite secretion. Thus, we conclude that coatings from tailored pectins show different biological activities in vitro and are potential innovative candidates for improving the biocompatibility of medical devices in various applications. © 2007 Wiley Periodicals.

AB - Previous work has reported the results of a multidisciplinary effort producing a proof-of-concept on the use of pectic polysaccharides in the surface modification of medical devices. This study was designed to learn more about the capability of engineered rhamnogalacturonan-I (RG-I) fractions of apple pectin to control bone cell and macrophage behavior. Thermanox® or polystyrene Petri dishes were surface modified with two different modified hairy regions (MHRs) obtained by different enzymatic liquefaction processes of apples differing in relative amounts and lengths of their neutral side chains: (long-haired) MHR-α and (short-haired) MHR-B. Bone expiants from 14-day-old chick embryos were cultured for 14 days on both pectic substrata. MHR-B promoted cell migration and differentiation, MHR-α did not. On MHR-α, J774.2 macrophages grew well, their percentage in G1 phase was decreased and in S phase increased, and they did not secrete either proinflammatory-cytokines or nitrites. Contrasting results were gained from macrophages on MHR-B, except for nitrite secretion. Thus, we conclude that coatings from tailored pectins show different biological activities in vitro and are potential innovative candidates for improving the biocompatibility of medical devices in various applications. © 2007 Wiley Periodicals.

KW - Biocompatibility

KW - Biomaterials

KW - Biomimetic surfaces

KW - Cell-surface interaction

KW - Rhamnogalacturonan-I

U2 - 10.1002/jbm.a.31729

DO - 10.1002/jbm.a.31729

M3 - Article

SN - 1549-3296

VL - 86

SP - 597

EP - 606

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

IS - 3

ER -

Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins

Abstract

Keywords

Access to Document

Fingerprint

Cite this