Molecular analysis of a leu2- mutant of Candida maltosa demonstrates the presence of multiple alleles

D. Becher; S. Schulze; A. Kasuske; H. Schulze; I. A. Samsonova; S. G. Oliver

doi:10.1007/BF00309549

Molecular analysis of a leu2- mutant of Candida maltosa demonstrates the presence of multiple alleles

D. Becher, S. Schulze, A. Kasuske, H. Schulze, I. A. Samsonova, S. G. Oliver

Research output: Contribution to journal › Article › peer-review

Abstract

Three different alleles of the β-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy-prototrophy-auxotrophy sequence of mutations in C. maltosa is suggested.

Original language	English
Pages (from-to)	208-216
Number of pages	8
Journal	Current Genetics
Volume	26
Issue number	3
DOIs	https://doi.org/10.1007/BF00309549
Publication status	Published - 1994

Keywords

β-isopropylmalate dehydrogenase
Allelic variation
Candida maltosa
Genome organisation
Silent genes

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

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10.1007/BF00309549

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@article{e4b689787cad483fa9e7f533ea3259ee,

title = "Molecular analysis of a leu2- mutant of Candida maltosa demonstrates the presence of multiple alleles",

abstract = "Three different alleles of the β-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy-prototrophy-auxotrophy sequence of mutations in C. maltosa is suggested.",

keywords = "β-isopropylmalate dehydrogenase, Allelic variation, Candida maltosa, Genome organisation, Silent genes",

author = "D. Becher and S. Schulze and A. Kasuske and H. Schulze and Samsonova, {I. A.} and Oliver, {S. G.}",

year = "1994",

doi = "10.1007/BF00309549",

language = "English",

volume = "26",

pages = "208--216",

journal = "Current Genetics",

issn = "0172-8083",

publisher = "Springer Nature",

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TY - JOUR

T1 - Molecular analysis of a leu2- mutant of Candida maltosa demonstrates the presence of multiple alleles

AU - Becher, D.

AU - Schulze, S.

AU - Kasuske, A.

AU - Schulze, H.

AU - Samsonova, I. A.

AU - Oliver, S. G.

PY - 1994

Y1 - 1994

N2 - Three different alleles of the β-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy-prototrophy-auxotrophy sequence of mutations in C. maltosa is suggested.

AB - Three different alleles of the β-isopropylmalate dehydrogenase gene were cloned and sequenced from a leucine auxotrophic mutant, G587, of Candida maltosa. The cloning of functionally-intact wild-type genes from this mutant strain suggests the presence of silent gene copies. An interallelic-divergence comparison has provided evidence for new regulatory mechanisms. Sequence data and karyotype analysis argue for a highly-aneuploid genome of C. maltosa. An interpretation for the spontaneous auxotrophy-prototrophy-auxotrophy sequence of mutations in C. maltosa is suggested.

KW - β-isopropylmalate dehydrogenase

KW - Allelic variation

KW - Candida maltosa

KW - Genome organisation

KW - Silent genes

U2 - 10.1007/BF00309549

DO - 10.1007/BF00309549

M3 - Article

C2 - 7859302

SN - 0172-8083

VL - 26

SP - 208

EP - 216

JO - Current Genetics

JF - Current Genetics

IS - 3

ER -

Molecular analysis of a leu2- mutant of Candida maltosa demonstrates the presence of multiple alleles

Abstract

Keywords

Research Beacons, Institutes and Platforms

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