Molecular cloning, lentiviral transduction, and expression of recombinant ADAMTSL2 and ADAMTSL4

Mukti Singh; Stuart A. Cain; Clair Baldock

doi:10.1007/978-1-4939-9698-8_12

Molecular cloning, lentiviral transduction, and expression of recombinant ADAMTSL2 and ADAMTSL4

Mukti Singh, Stuart A. Cain, Clair Baldock^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Lentiviral systems have proven advantageous in the delivery and long-term integration of gene sequences into the genome of several cell types in vitro, in vivo, as well as in clinical trials. Here we detail the protocols involved in the molecular cloning of ADAMTSL2 and ADAMTSL4 into the human immunodeficiency virus (HIV)-derived pCDH lentiviral system. We also describe the lentiviral transduction of ADAMTSL2 and ADAMTSL4 into mammalian HEK293-EBNA cells to create stable cell lines, as well as their recombinant expression.

Original language	English
Title of host publication	ADAMTS proteases
Subtitle of host publication	methods in molecular biology
Place of Publication	New York
Publisher	Humana Press, Inc
Pages	137-155
Number of pages	19
Volume	2043
ISBN (Electronic)	9781493996988
ISBN (Print)	9781493996971
DOIs	https://doi.org/10.1007/978-1-4939-9698-8_12
Publication status	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2043
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

HEK293-EBNA
Lentiviral system
Lentiviral transduction
Mammalian
Molecular cloning
Recombinant expression
Stable cell lines

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-9698-8_12

Cite this

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abstract = "Lentiviral systems have proven advantageous in the delivery and long-term integration of gene sequences into the genome of several cell types in vitro, in vivo, as well as in clinical trials. Here we detail the protocols involved in the molecular cloning of ADAMTSL2 and ADAMTSL4 into the human immunodeficiency virus (HIV)-derived pCDH lentiviral system. We also describe the lentiviral transduction of ADAMTSL2 and ADAMTSL4 into mammalian HEK293-EBNA cells to create stable cell lines, as well as their recombinant expression.",

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Molecular cloning, lentiviral transduction, and expression of recombinant ADAMTSL2 and ADAMTSL4. / Singh, Mukti; Cain, Stuart A.; Baldock, Clair.
ADAMTS proteases: methods in molecular biology. Vol. 2043 New York: Humana Press, Inc, 2020. p. 137-155 (Methods in Molecular Biology; Vol. 2043).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Singh, Mukti

AU - Cain, Stuart A.

AU - Baldock, Clair

PY - 2020

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N2 - Lentiviral systems have proven advantageous in the delivery and long-term integration of gene sequences into the genome of several cell types in vitro, in vivo, as well as in clinical trials. Here we detail the protocols involved in the molecular cloning of ADAMTSL2 and ADAMTSL4 into the human immunodeficiency virus (HIV)-derived pCDH lentiviral system. We also describe the lentiviral transduction of ADAMTSL2 and ADAMTSL4 into mammalian HEK293-EBNA cells to create stable cell lines, as well as their recombinant expression.

AB - Lentiviral systems have proven advantageous in the delivery and long-term integration of gene sequences into the genome of several cell types in vitro, in vivo, as well as in clinical trials. Here we detail the protocols involved in the molecular cloning of ADAMTSL2 and ADAMTSL4 into the human immunodeficiency virus (HIV)-derived pCDH lentiviral system. We also describe the lentiviral transduction of ADAMTSL2 and ADAMTSL4 into mammalian HEK293-EBNA cells to create stable cell lines, as well as their recombinant expression.

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KW - Lentiviral system

KW - Lentiviral transduction

KW - Mammalian

KW - Molecular cloning

KW - Recombinant expression

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Molecular cloning, lentiviral transduction, and expression of recombinant ADAMTSL2 and ADAMTSL4

Abstract

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Keywords

UN SDGs

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