Molecular cloning, lentiviral transduction, and expression of recombinant ADAMTSL2 and ADAMTSL4

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Lentiviral systems have proven advantageous in the delivery and long-term integration of gene sequences into the genome of several cell types in vitro, in vivo, as well as in clinical trials. Here we detail the protocols involved in the molecular cloning of ADAMTSL2 and ADAMTSL4 into the human immunodeficiency virus (HIV)-derived pCDH lentiviral system. We also describe the lentiviral transduction of ADAMTSL2 and ADAMTSL4 into mammalian HEK293-EBNA cells to create stable cell lines, as well as their recombinant expression.
Original languageEnglish
Title of host publicationADAMTS proteases
Subtitle of host publicationmethods in molecular biology
Place of PublicationNew York
PublisherHumana Press, Inc
Pages137-155
Number of pages19
Volume2043
ISBN (Electronic)9781493996988
ISBN (Print)9781493996971
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2043
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • HEK293-EBNA
  • Lentiviral system
  • Lentiviral transduction
  • Mammalian
  • Molecular cloning
  • Recombinant expression
  • Stable cell lines

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