Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

A. P M Jongsma; P. Kenemans; J. M J Piek; R. P. Zweemer; R. H M Verheijen; J. W T Klein Gebbinck; G. J. Van Kamp; I. J. Jacobs; P. Shaw; P. J. Van Diest

doi:10.1136/mp.55.5.305

Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

A. P M Jongsma, P. Kenemans, J. M J Piek, R. P. Zweemer, R. H M Verheijen, J. W T Klein Gebbinck, G. J. Van Kamp, I. J. Jacobs, P. Shaw, P. J. Van Diest

Research output: Contribution to journal › Article › peer-review

Abstract

Background/Aims: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer. Materials/methods: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q. Results: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases. Conclusion: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.

Original language	English
Pages (from-to)	305-309
Number of pages	4
Journal	Journal of Clinical Pathology
Volume	55
Issue number	5
DOIs	https://doi.org/10.1136/mp.55.5.305
Publication status	Published - Oct 2002

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Jongsma, A. P. M., Kenemans, P., Piek, J. M. J., Zweemer, R. P., Verheijen, R. H. M., Klein Gebbinck, J. W. T., Van Kamp, G. J., Jacobs, I. J., Shaw, P., & Van Diest, P. J. (2002). Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer. Journal of Clinical Pathology, 55(5), 305-309. https://doi.org/10.1136/mp.55.5.305

@article{581f0bb48f1841a29a2565478b4980e9,

title = "Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer",

abstract = "Background/Aims: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer. Materials/methods: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q. Results: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases. Conclusion: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.",

author = "Jongsma, {A. P M} and P. Kenemans and Piek, {J. M J} and Zweemer, {R. P.} and Verheijen, {R. H M} and {Klein Gebbinck}, {J. W T} and {Van Kamp}, {G. J.} and Jacobs, {I. J.} and P. Shaw and {Van Diest}, {P. J.}",

year = "2002",

month = oct,

doi = "10.1136/mp.55.5.305",

language = "English",

volume = "55",

pages = "305--309",

journal = "Journal of Clinical Pathology",

issn = "1472-4146",

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Jongsma, APM, Kenemans, P, Piek, JMJ, Zweemer, RP, Verheijen, RHM, Klein Gebbinck, JWT, Van Kamp, GJ, Jacobs, IJ, Shaw, P & Van Diest, PJ 2002, 'Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer', Journal of Clinical Pathology, vol. 55, no. 5, pp. 305-309. https://doi.org/10.1136/mp.55.5.305

TY - JOUR

T1 - Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

AU - Jongsma, A. P M

AU - Kenemans, P.

AU - Piek, J. M J

AU - Zweemer, R. P.

AU - Verheijen, R. H M

AU - Klein Gebbinck, J. W T

AU - Van Kamp, G. J.

AU - Jacobs, I. J.

AU - Shaw, P.

AU - Van Diest, P. J.

PY - 2002/10

Y1 - 2002/10

N2 - Background/Aims: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer. Materials/methods: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q. Results: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases. Conclusion: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.

AB - Background/Aims: Loss of heterozygosity (LOH) on chromosome 13q has been reported to occur frequently in human ovarian cancer, and indications have been found that chromosome 13 may also play a specific role in the inherited form of ovarian cancer. The aim of this study was to define regions on chromosome 13 that may harbour additional tumour suppressor genes involved in the tumorigenesis of BRCA1 related ovarian and fallopian tube cancer. Materials/methods: DNA extracted from paraffin wax blocks of 36 BRCA1 associated ovarian and fallopian tube carcinomas was analysed by LOH polymerase chain reaction using seven highly polymorphic microsatellite markers spanning chromosome 13q. Results: High LOH frequencies were found on loci 13q11, 13q14, 13q21, 13q22-31, 13q32, and 13q32-4, suggesting the presence of putative tumour suppressor genes on the long arm of chromosome 13 that may play a role in the pathogenesis of BRCA1 related ovarian and fallopian tube cancer. LOH patterns appeared to be independent of the type of BRCA1 mutation, stage, and grade. Although in some cases there were indications for loss of larger parts of chromosome 13, in most cases losses were fairly randomly distributed over chromosome 13 with retained parts in between lost parts. Microsatellite instability was found in six cases. Conclusion: Several loci on chromosome 13q show high frequencies of LOH in BRCA1 related ovarian and fallopian tube cancer, and may therefore harbour putative tumour suppressor genes involved in the carcinogenesis of this particular type of hereditary cancer.

U2 - 10.1136/mp.55.5.305

DO - 10.1136/mp.55.5.305

M3 - Article

C2 - 12354934

SN - 1472-4146

VL - 55

SP - 305

EP - 309

JO - Journal of Clinical Pathology

JF - Journal of Clinical Pathology

IS - 5

ER -

Molecular evidence for putative tumour suppressor genes on chromosome 13q specific to BRCA1 related ovarian and fallopian tube cancer

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