Monoclonal antibodies to testosterone: The effect of immunogen structure on specificity

A. White; C. Gray; J. E T Corrie

Monoclonal antibodies to testosterone: The effect of immunogen structure on specificity

A. White, C. Gray, J. E T Corrie

Research output: Contribution to journal › Article › peer-review

Abstract

A 15β-thioalkyl derivative of testosterone conjugated to bovine serum albumin (BSA) was synthesised and used to produce monoclonal antibodies. These antibodies were evaluated using 15β-(2-carboxyphenylthio)-testosterone [125I]histamine as radioligand. Out of 1368 hybrids, 5 secreted anti-testosterone antibodies. These were compared with monoclonal antibodies derived from immunisation with testosterone-3-carboxymethyloxime-BSA. The first group of monoclonal antibodies all showed very low cross-reactivity with 5α-dihydrotestosterone (

Original language	English
Pages (from-to)	169-175
Number of pages	6
Journal	Journal of Steroid Biochemistry
Volume	22
Issue number	2
Publication status	Published - Feb 1985

Cite this

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title = "Monoclonal antibodies to testosterone: The effect of immunogen structure on specificity",

abstract = "A 15β-thioalkyl derivative of testosterone conjugated to bovine serum albumin (BSA) was synthesised and used to produce monoclonal antibodies. These antibodies were evaluated using 15β-(2-carboxyphenylthio)-testosterone [125I]histamine as radioligand. Out of 1368 hybrids, 5 secreted anti-testosterone antibodies. These were compared with monoclonal antibodies derived from immunisation with testosterone-3-carboxymethyloxime-BSA. The first group of monoclonal antibodies all showed very low cross-reactivity with 5α-dihydrotestosterone (",

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year = "1985",

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AU - White, A.

AU - Gray, C.

AU - Corrie, J. E T

PY - 1985/2

Y1 - 1985/2

N2 - A 15β-thioalkyl derivative of testosterone conjugated to bovine serum albumin (BSA) was synthesised and used to produce monoclonal antibodies. These antibodies were evaluated using 15β-(2-carboxyphenylthio)-testosterone [125I]histamine as radioligand. Out of 1368 hybrids, 5 secreted anti-testosterone antibodies. These were compared with monoclonal antibodies derived from immunisation with testosterone-3-carboxymethyloxime-BSA. The first group of monoclonal antibodies all showed very low cross-reactivity with 5α-dihydrotestosterone (

AB - A 15β-thioalkyl derivative of testosterone conjugated to bovine serum albumin (BSA) was synthesised and used to produce monoclonal antibodies. These antibodies were evaluated using 15β-(2-carboxyphenylthio)-testosterone [125I]histamine as radioligand. Out of 1368 hybrids, 5 secreted anti-testosterone antibodies. These were compared with monoclonal antibodies derived from immunisation with testosterone-3-carboxymethyloxime-BSA. The first group of monoclonal antibodies all showed very low cross-reactivity with 5α-dihydrotestosterone (

M3 - Article

SN - 0022-4731

VL - 22

SP - 169

EP - 175

JO - Journal of Steroid Biochemistry

JF - Journal of Steroid Biochemistry

IS - 2

ER -

Monoclonal antibodies to testosterone: The effect of immunogen structure on specificity

Abstract

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