Monocyte-derived dendritic cells perform hemophagocytosis to fine-tune excessive immune responses

Hideaki Ohyagi, Nobuyuki Onai, Taku Sato, Satoshi Yotsumoto, Jiajia Liu, Hisaya Akiba, Hideo Yagita, Koji Atarashi, Kenya Honda, Axel Roers, Werner Müller, Kazutaka Kurabayashi, Mayuka Hosoi-Amaike, Naoto Takahashi, Makoto Hirokawa, Kouji Matsushima, Kenichi Sawada, Toshiaki Ohteki

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Because immune responses simultaneously defend and injure the host, the immune system must be finely regulated to ensure the host's survival. Here, we have shown that when injected with high Toll-like receptor ligand doses or infected with lymphocytic choriomeningitis virus (LCMV) clone 13, which has a high viral turnover, inflammatory monocyte-derived dendritic cells (Mo-DCs) engulfed apoptotic erythroid cells. In this process, called hemophagocytosis, phosphatidylserine (PS) served as an "eat-me" signal. Type I interferons were necessary for both PS exposure on erythroid cells and the expression of PS receptors in the Mo-DCs. Importantly, hemophagocytosis was required for interleukin-10 (IL-10) production from Mo-DCs. Blocking hemophagocytosis or Mo-DC-derived IL-10 significantly increased cytotoxic Tcell lymphocyte activity, tissue damage, and mortality in virus-infected hosts, suggesting that hemophagocytosis moderates immune responses to ensure the host's survival invivo. This sheds light on the physiological relevance of hemophagocytosis in severe inflammatory and infectious diseases. © 2013 Elsevier Inc.
    Original languageEnglish
    Pages (from-to)584-598
    Number of pages14
    JournalImmunity
    Volume39
    Issue number3
    DOIs
    Publication statusPublished - 19 Sept 2013

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