Morpholino oligonucleotide crosslinked hydrogels as portable optical oligonucleotide biosensors

Geraint Langford; Jaclyn Raeburn; David Ferrier; Philip Hands; Michael Shaver

doi:10.1021/acssensors.8b01208

Morpholino oligonucleotide crosslinked hydrogels as portable optical oligonucleotide biosensors

Geraint Langford
, Jaclyn Raeburn
, David Ferrier
, Philip Hands
, Michael Shaver

Research output: Contribution to journal › Article › peer-review

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Abstract

Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

Original language	English
Pages (from-to)	185-191
Number of pages	7
Journal	ACS Sensors
Volume	4
Issue number	1
DOIs	https://doi.org/10.1021/acssensors.8b01208
Publication status	Published - 28 Dec 2018

Keywords

biosensor
hydrogel
microRNA
morpholino oligonucleotide
oligonucleotide sensor

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10.1021/acssensors.8b01208

MOCHS - ACS - R v4.1Accepted author manuscript, 4.42 MB

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title = "Morpholino oligonucleotide crosslinked hydrogels as portable optical oligonucleotide biosensors",

abstract = "Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.",

keywords = "biosensor, hydrogel, microRNA, morpholino oligonucleotide, oligonucleotide sensor",

author = "Geraint Langford and Jaclyn Raeburn and David Ferrier and Philip Hands and Michael Shaver",

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doi = "10.1021/acssensors.8b01208",

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AU - Langford, Geraint

AU - Raeburn, Jaclyn

AU - Ferrier, David

AU - Hands, Philip

AU - Shaver, Michael

N1 - Funding Information: We would like to thank the Engineering and Physical Sciences Research Council (EPSRC) and Axis-Shield Diagnostics Ltd. for a CASE studentship for GL and the MRC CiC programme for earlier financial support of this project. Publisher Copyright: © 2018 American Chemical Society. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2018/12/28

Y1 - 2018/12/28

N2 - Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

AB - Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive crosslinks for microRNA sequence detection. The MO crosslinks can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO crosslinks offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

KW - biosensor

KW - hydrogel

KW - microRNA

KW - morpholino oligonucleotide

KW - oligonucleotide sensor

UR - https://www.scopus.com/pages/publications/85060022681

UR - http://www.mendeley.com/research/morpholino-oligonucleotide-crosslinked-hydrogels-portable-optical-oligonucleotide-biosensors

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JO - ACS Sensors

JF - ACS Sensors

IS - 1

ER -

Morpholino oligonucleotide crosslinked hydrogels as portable optical oligonucleotide biosensors

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Keywords

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