Abstract
T1-weighted NMR imaging of the isolated perfused rat hippocampal slice was used to estimate cell volume fraction. Eight brain slices were studied in artificial cerebrospinal fluid (aCSF) using a 600 MHz narrow bore spectrometer and a home built perfusion chamber. Cell volume fraction was calculated as 1 - f(ECS), where f(ECS) is the distribution volume of gadodiamide in the slice. This was determined by measuring the T1 of the slice before and after perfusion with gadodiamide. A mean cell volume fraction of 0.66 ± 0.04 was estimated. The addition of 60 mM mannitol to three of the brain slices produced a 26% decrease in the cell volume fraction. The technique affords a simple means of estimating cell volume fraction and can be extended to produce images reflecting cell density.
| Original language | English |
|---|---|
| Pages (from-to) | 603-607 |
| Number of pages | 4 |
| Journal | Magnetic Resonance in Medicine |
| Volume | 42 |
| Issue number | 3 |
| Publication status | Published - 1999 |
Keywords
- Brain slice
- Cell volume
- Gadolinium
- T1 relaxation