MRI measurement of cell volume fraction in the perfused rat hippocampal slice

David Buckley, David L. Buckley, Bui Jonathan D, M. Ian Phillips, Stephen J. Blackband

    Research output: Contribution to journalArticlepeer-review


    T1-weighted NMR imaging of the isolated perfused rat hippocampal slice was used to estimate cell volume fraction. Eight brain slices were studied in artificial cerebrospinal fluid (aCSF) using a 600 MHz narrow bore spectrometer and a home built perfusion chamber. Cell volume fraction was calculated as 1 - f(ECS), where f(ECS) is the distribution volume of gadodiamide in the slice. This was determined by measuring the T1 of the slice before and after perfusion with gadodiamide. A mean cell volume fraction of 0.66 ± 0.04 was estimated. The addition of 60 mM mannitol to three of the brain slices produced a 26% decrease in the cell volume fraction. The technique affords a simple means of estimating cell volume fraction and can be extended to produce images reflecting cell density.
    Original languageEnglish
    Pages (from-to)603-607
    Number of pages4
    JournalMagnetic Resonance in Medicine
    Issue number3
    Publication statusPublished - 1999


    • Brain slice
    • Cell volume
    • Gadolinium
    • T1 relaxation


    Dive into the research topics of 'MRI measurement of cell volume fraction in the perfused rat hippocampal slice'. Together they form a unique fingerprint.

    Cite this