mRNA localization to P-bodies in yeast is bi-phasic with many mRNAs captured in a late Bfr1p-dependent wave

Clare E. Simpson, Jennifer Lui, Christopher J. Kershaw, Paul F G Sims, Mark P. Ashe

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The relocalization of translationally repressed mRNAs to mRNA processing bodies (P-bodies) is a key consequence of cellular stress across many systems. P-bodies harbor mRNA degradation components and are implicated in mRNA decay, but the relative timing and control of mRNA relocalization to P-bodies is poorly understood. We used the MS2-GFP system to follow the movement of specific endogenous mRNAs in live Saccharomyces cerevisiae cells after nutritional stress. It appears that the relocalization of mRNA to P-bodies after stress is bi-phasic: some mRNAs are present early, whereas others are recruited much later concomitant with recruitment of translation initiation factors, such as eIF4E. We also find that Bfr1p is a late-phase-localizing P-body protein that is important for the delayed entry of certain mRNAS to P-bodies. Therefore, for the mRNAs tested, relocalization to P-bodies varies both in terms of the kinetics and factor requirements. This work highlights a potential new regulatory juncture in gene expression that would facilitate the overall rationalization of protein content required for adaptation to stress. © 2014. Published by The Company of Biologists Ltd.
    Original languageEnglish
    Pages (from-to)1254-1262
    Number of pages8
    JournalJournal of Cell Science
    Volume127
    DOIs
    Publication statusPublished - Mar 2014

    Keywords

    • Glucose regulation
    • MRNA localization
    • P-bodies
    • Stress granules
    • Yeast

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