TY - JOUR
T1 - Multiple P2Y receptors couple to calcium-dependent, Chloride channels in smooth muscle cells of the rat pulmonary artery
AU - Chootip, Krongkarn
AU - Gurney, Alison M.
AU - Kennedy, Charles
PY - 2005/10/26
Y1 - 2005/10/26
N2 - Background: Uridine 5′-triphosphate (UTP) and uridine 5′-diphosphate (UDP) act via P2Y receptors to evoke contraction of rat pulmonary arteries, whilst adenosine 5′-triphosphate (ATP) acts via P2X and P2Y receptors. Pharmacological characterisation of these receptors in intact arteries is complicated by release and extracellular metabolism of nucleotides, so the aim of this study was to characterise the P2Y receptors under conditions that minimise these problems. Methods: The perforated-patch clamp technique was used to record the Ca2+-dependent, Cl- current (ICl,Ca) activated by P2Y receptor agonists in acutely dissociated smooth muscle cells of rat small (SPA) and large (LPA) intrapulmonary arteries, held at -50 mV. Contractions to ATP were measured in isolated muscle rings. Data were compared by Student's t test or one way ANOVA. Results: ATP, UTP and UDP (10-4M) evoked oscillating, inward currents (peak = 13-727 pA) in 71-93% of cells. The first current was usually the largest and in the SPA the response to ATP was significantly greater than those to UTP or UDP (P
AB - Background: Uridine 5′-triphosphate (UTP) and uridine 5′-diphosphate (UDP) act via P2Y receptors to evoke contraction of rat pulmonary arteries, whilst adenosine 5′-triphosphate (ATP) acts via P2X and P2Y receptors. Pharmacological characterisation of these receptors in intact arteries is complicated by release and extracellular metabolism of nucleotides, so the aim of this study was to characterise the P2Y receptors under conditions that minimise these problems. Methods: The perforated-patch clamp technique was used to record the Ca2+-dependent, Cl- current (ICl,Ca) activated by P2Y receptor agonists in acutely dissociated smooth muscle cells of rat small (SPA) and large (LPA) intrapulmonary arteries, held at -50 mV. Contractions to ATP were measured in isolated muscle rings. Data were compared by Student's t test or one way ANOVA. Results: ATP, UTP and UDP (10-4M) evoked oscillating, inward currents (peak = 13-727 pA) in 71-93% of cells. The first current was usually the largest and in the SPA the response to ATP was significantly greater than those to UTP or UDP (P
U2 - 10.1186/1465-9921-6-124
DO - 10.1186/1465-9921-6-124
M3 - Article
C2 - 16250909
SN - 1465-9921
VL - 6
JO - Respiratory research
JF - Respiratory research
M1 - 124
ER -
