TY - JOUR
T1 - Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells
AU - Pascual-Reguant, Anna
AU - Köhler, Ralf
AU - Mothes, Ronja
AU - Bauherr, Sandy
AU - Hernández, Daniela C
AU - Uecker, Ralf
AU - Holzwarth, Karolin
AU - Kotsch, Katja
AU - Seidl, Maximilian
AU - Philipsen, Lars
AU - Müller, Werner
AU - Romagnani, Chiara
AU - Niesner, Raluca
AU - Hauser, Anja E
N1 - Funding Information:
We would like to thank Daniel Wendisch (Immunology of Infectious Diseases & Vac-cinology group, Charité—Universitätsmedizin, Berlin) for helpful discussions. This study was supported by funding from the Deutsche Forschungsgemeinschaft, (SPP1937, HA5354/8-2 to A.E.H.), TRR 130 C01 and SFB 1444 P14, to A.E.H. and R.N. The contribution of Max Seidl was funded in part through CRC 1160 traveling grants. L.P. was supported by CRC854, Z01. The work was supported by an “Adding 3R Value” grant of Charité 3R to A.E.H. The authors affiliated with Charité for the duration of this study acknowledge that Charité is a corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin.
Publisher Copyright:
© 2021, The Author(s).
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/3/19
Y1 - 2021/3/19
N2 - Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127+ ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127+ ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology.
AB - Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127+ ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127+ ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology.
U2 - 10.1038/s41467-021-21994-8
DO - 10.1038/s41467-021-21994-8
M3 - Article
C2 - 33741932
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1737
ER -
