Murine AGM single-cell profiling identifies a continuum of hemogenic endothelium differentiation marked by ACE

Muhammad Zaki Hidayatullah Fadlullah, Wen Hao Neo, Michael Lie-a-ling, Roshana Thambyrajah, Rahima Patel, Renaud Mevel, Irène Aksoy, Nam Do Khoa, Pierre Savatier, Laura Fontenille, Syed Murtuza Baker, Magnus Rattray, Valerie Kouskoff, Georges Lacaud

Research output: Contribution to journalArticlepeer-review

90 Downloads (Pure)


In vitro generation and expansion of hematopoietic stem cells (HSCs) holds great promise for the treatment of any ailment that relies on bone marrow or blood transplantation. To achieve this, it is essential to resolve the molecular and cellular pathways that govern HSC formation in the embryo. HSCs first emerge in the aorta-gonad-mesonephros (AGM) region, where a rare subset of endothelial cells, hemogenic endothelium (HE), undergoes an endothelial-to-hematopoietic transition (EHT). Here, we present full-length single-cell RNA sequencing (scRNA-seq) of the EHT process with a focus on HE and dorsal aorta niche cells. By using Runx1b and Gfi1/1b transgenic reporter mouse models to isolate HE, we uncovered that the pre-HE to HE continuum is specifically marked by angiotensin-I converting enzyme (ACE) expression. We established that HE cells begin to enter the cell cycle near the time of EHT initiation when their morphology still resembles endothelial cells. We further demonstrated that RUNX1 AGM niche cells consist of vascular smooth muscle cells and PDGFRa+ mesenchymal cells and can functionally support hematopoiesis. Overall, our study provides new insights into HE differentiation toward HSC and the role of AGM RUNX1+ niche cells in this process. Our expansive scRNA-seq datasets represents a powerful resource to investigate these processes further.

Original languageEnglish
Pages (from-to)343-356
Number of pages14
Issue number3
Early online date15 Sept 2021
Publication statusPublished - 20 Jan 2022


  • Animals
  • Cell Differentiation
  • Embryo, Mammalian/cytology
  • Hemangioblasts/cytology
  • Hematopoiesis
  • Hematopoietic Stem Cells/cytology
  • Mesonephros/cytology
  • Mice
  • Single-Cell Analysis
  • Transcriptome
  • Zebrafish

Research Beacons, Institutes and Platforms

  • Manchester Cancer Research Centre


Dive into the research topics of 'Murine AGM single-cell profiling identifies a continuum of hemogenic endothelium differentiation marked by ACE'. Together they form a unique fingerprint.

Cite this