Mutants of Aspergillus nidulans with increased resistance to the alkylating agent, N-methyl-N′-nitro-N-nitrosoguanidine

P. Hooley; S. G. Shawcross; P. Strike

doi:10.1016/0167-8817(88)90035-1

Mutants of Aspergillus nidulans with increased resistance to the alkylating agent, N-methyl-N′-nitro-N-nitrosoguanidine

P. Hooley, S. G. Shawcross^*, P. Strike

^*Corresponding author for this work

FBMH Faculty Office Administration (L5)

University of Liverpool

Research output: Contribution to journal › Article › peer-review

Abstract

The isolation and characterisation of mutants of Aspergillus nidulans showing resistance to MNNG is described. Such isolates were stable through prolonged subculture in the absence of the selective agent, and resistance segregated as an allele of a single gene in meiotic and mitotic analysis. MNNG-resistant strains showed an increase in resistance to EMS and UV irradiation but no cross-resistance to MMS was detected. Possible mechanisms of resistance to alkylating agents are discussed.

Original language	English
Pages (from-to)	247-254
Number of pages	8
Journal	Mutation Research DNA Repair Reports
Volume	193
Issue number	3
DOIs	https://doi.org/10.1016/0167-8817(88)90035-1
Publication status	Published - 1988

Keywords

Alkylating agents,resistance to
Aspergillus nidulans
DNA repair
MNNG resistance

Access to Document

10.1016/0167-8817(88)90035-1

Cite this

@article{ca8f1f4041b74f3e86f18272fda82e86,

title = "Mutants of Aspergillus nidulans with increased resistance to the alkylating agent, N-methyl-N′-nitro-N-nitrosoguanidine",

abstract = "The isolation and characterisation of mutants of Aspergillus nidulans showing resistance to MNNG is described. Such isolates were stable through prolonged subculture in the absence of the selective agent, and resistance segregated as an allele of a single gene in meiotic and mitotic analysis. MNNG-resistant strains showed an increase in resistance to EMS and UV irradiation but no cross-resistance to MMS was detected. Possible mechanisms of resistance to alkylating agents are discussed.",

keywords = "Alkylating agents,resistance to, Aspergillus nidulans, DNA repair, MNNG resistance",

author = "P. Hooley and Shawcross, {S. G.} and P. Strike",

year = "1988",

doi = "10.1016/0167-8817(88)90035-1",

language = "English",

volume = "193",

pages = "247--254",

journal = "Mutation Research DNA Repair Reports",

issn = "0167-8817",

publisher = "Elsevier BV",

number = "3",

}

TY - JOUR

T1 - Mutants of Aspergillus nidulans with increased resistance to the alkylating agent, N-methyl-N′-nitro-N-nitrosoguanidine

AU - Hooley, P.

AU - Shawcross, S. G.

AU - Strike, P.

PY - 1988

Y1 - 1988

N2 - The isolation and characterisation of mutants of Aspergillus nidulans showing resistance to MNNG is described. Such isolates were stable through prolonged subculture in the absence of the selective agent, and resistance segregated as an allele of a single gene in meiotic and mitotic analysis. MNNG-resistant strains showed an increase in resistance to EMS and UV irradiation but no cross-resistance to MMS was detected. Possible mechanisms of resistance to alkylating agents are discussed.

AB - The isolation and characterisation of mutants of Aspergillus nidulans showing resistance to MNNG is described. Such isolates were stable through prolonged subculture in the absence of the selective agent, and resistance segregated as an allele of a single gene in meiotic and mitotic analysis. MNNG-resistant strains showed an increase in resistance to EMS and UV irradiation but no cross-resistance to MMS was detected. Possible mechanisms of resistance to alkylating agents are discussed.

KW - Alkylating agents,resistance to

KW - Aspergillus nidulans

KW - DNA repair

KW - MNNG resistance

UR - http://www.scopus.com/inward/record.url?scp=0023884151&partnerID=8YFLogxK

U2 - 10.1016/0167-8817(88)90035-1

DO - 10.1016/0167-8817(88)90035-1

M3 - Article

C2 - 3283539

AN - SCOPUS:0023884151

SN - 0167-8817

VL - 193

SP - 247

EP - 254

JO - Mutation Research DNA Repair Reports

JF - Mutation Research DNA Repair Reports

IS - 3

ER -

Mutants of Aspergillus nidulans with increased resistance to the alkylating agent, N-methyl-N′-nitro-N-nitrosoguanidine

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this