Mutation of a single amino acid residue in the basic region of the Epstein-Barr virus (EBV) lytic cycle switch protein Zta (BZLF1) prevents reactivation of EBV from latency

Celine Schelcher; Celine Scheicher; Sarah Valencia; Henri Jacques Delecluse; Matthew Hicks; Alison J. Sinclair

doi:10.1128/JVI.79.21.13822-13828.2005

Mutation of a single amino acid residue in the basic region of the Epstein-Barr virus (EBV) lytic cycle switch protein Zta (BZLF1) prevents reactivation of EBV from latency

Celine Schelcher
, Celine Scheicher
, Sarah Valencia
, Henri Jacques Delecluse
, Matthew Hicks
, Alison J. Sinclair

Research output: Contribution to journal › Article › peer-review

Abstract

Zta, the product of the BZLF1 gene carried by Epstein-Barr virus (EBV), is crucial for reactivation of EBV from latency. Zta is a member of the bZIP family of transcription factors, and in common with many of these, Zta possesses a conserved cysteine residue in its basic region (C189) and a further cysteine residue in its ZIP region (C222). We demonstrate that C189 is required to reactivate EBV from latency but C222 is not and that this single amino acid affects two independent functions of Zta, (i) binding to a Zta-responsive site and (ii) manipulating the cell cycle. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Original language	English
Pages (from-to)	13822-13828
Number of pages	6
Journal	Journal of virology
Volume	79
Issue number	21
DOIs	https://doi.org/10.1128/JVI.79.21.13822-13828.2005
Publication status	Published - Nov 2005

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

physiology: Cell Cycle
Cell Line
genetics: Cysteine
chemistry: DNA-Binding Proteins
physiology: Herpesvirus 4, Human
Humans
Mutation
chemistry: Trans-Activators
chemistry: Viral Proteins
Virus Activation

Access to Document

10.1128/JVI.79.21.13822-13828.2005

Cite this

@article{f5066aca269b46f8a2776ed4d5e5f026,

title = "Mutation of a single amino acid residue in the basic region of the Epstein-Barr virus (EBV) lytic cycle switch protein Zta (BZLF1) prevents reactivation of EBV from latency",

abstract = "Zta, the product of the BZLF1 gene carried by Epstein-Barr virus (EBV), is crucial for reactivation of EBV from latency. Zta is a member of the bZIP family of transcription factors, and in common with many of these, Zta possesses a conserved cysteine residue in its basic region (C189) and a further cysteine residue in its ZIP region (C222). We demonstrate that C189 is required to reactivate EBV from latency but C222 is not and that this single amino acid affects two independent functions of Zta, (i) binding to a Zta-responsive site and (ii) manipulating the cell cycle. Copyright {\textcopyright} 2005, American Society for Microbiology. All Rights Reserved.",

keywords = "physiology: Cell Cycle, Cell Line, genetics: Cysteine, chemistry: DNA-Binding Proteins, physiology: Herpesvirus 4, Human, Humans, Mutation, chemistry: Trans-Activators, chemistry: Viral Proteins, Virus Activation",

author = "Celine Schelcher and Celine Scheicher and Sarah Valencia and Delecluse, \{Henri Jacques\} and Matthew Hicks and Sinclair, \{Alison J.\}",

year = "2005",

month = nov,

doi = "10.1128/JVI.79.21.13822-13828.2005",

language = "English",

volume = "79",

pages = "13822--13828",

journal = "Journal of virology",

issn = "1098-5514",

publisher = "American Society for Microbiology",

number = "21",

}

Mutation of a single amino acid residue in the basic region of the Epstein-Barr virus (EBV) lytic cycle switch protein Zta (BZLF1) prevents reactivation of EBV from latency. / Schelcher, Celine; Scheicher, Celine; Valencia, Sarah et al.
In: Journal of virology, Vol. 79, No. 21, 11.2005, p. 13822-13828.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Mutation of a single amino acid residue in the basic region of the Epstein-Barr virus (EBV) lytic cycle switch protein Zta (BZLF1) prevents reactivation of EBV from latency

AU - Schelcher, Celine

AU - Scheicher, Celine

AU - Valencia, Sarah

AU - Delecluse, Henri Jacques

AU - Hicks, Matthew

AU - Sinclair, Alison J.

PY - 2005/11

Y1 - 2005/11

N2 - Zta, the product of the BZLF1 gene carried by Epstein-Barr virus (EBV), is crucial for reactivation of EBV from latency. Zta is a member of the bZIP family of transcription factors, and in common with many of these, Zta possesses a conserved cysteine residue in its basic region (C189) and a further cysteine residue in its ZIP region (C222). We demonstrate that C189 is required to reactivate EBV from latency but C222 is not and that this single amino acid affects two independent functions of Zta, (i) binding to a Zta-responsive site and (ii) manipulating the cell cycle. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

AB - Zta, the product of the BZLF1 gene carried by Epstein-Barr virus (EBV), is crucial for reactivation of EBV from latency. Zta is a member of the bZIP family of transcription factors, and in common with many of these, Zta possesses a conserved cysteine residue in its basic region (C189) and a further cysteine residue in its ZIP region (C222). We demonstrate that C189 is required to reactivate EBV from latency but C222 is not and that this single amino acid affects two independent functions of Zta, (i) binding to a Zta-responsive site and (ii) manipulating the cell cycle. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

KW - physiology: Cell Cycle

KW - Cell Line

KW - genetics: Cysteine

KW - chemistry: DNA-Binding Proteins

KW - physiology: Herpesvirus 4, Human

KW - Humans

KW - Mutation

KW - chemistry: Trans-Activators

KW - chemistry: Viral Proteins

KW - Virus Activation

U2 - 10.1128/JVI.79.21.13822-13828.2005

DO - 10.1128/JVI.79.21.13822-13828.2005

M3 - Article

SN - 1098-5514

VL - 79

SP - 13822

EP - 13828

JO - Journal of virology

JF - Journal of virology

IS - 21

ER -

Mutation of a single amino acid residue in the basic region of the Epstein-Barr virus (EBV) lytic cycle switch protein Zta (BZLF1) prevents reactivation of EBV from latency

Abstract

UN SDGs

Keywords

Access to Document

Fingerprint

Cite this