Mutations in the waaR gene of Escherichia coli which disrupt lipopolysaccharide outer core biosynthesis affect cell surface retention of group 2 capsular polysaccharides

Clare M. Taylor, Marie Goldrick, Liz Lord, Ian S. Roberts

    Research output: Contribution to journalArticlepeer-review

    Abstract

    On the basis of increased resistance to KS capsule-specific bacteriophage, a waaR transposon mutant defective in the biosynthesis of lipopolysaccharide outer core was isolated. In a K1-expressing strain the mutation equally affected sensitivity to K1 capsule-specific bacteriophage, indicating a general effect on group 2 capsules. The waaR mutation affected retention on the cell surface of the K5 polysaccharide, with increased polysaccharide accumulating in the culture supernatant. This indicates that interactions between the outer core of lipopolysaccharide and group 2 capsular polysaccharides are important for the stabilization of group 2 capsular polysaccharides on the cell surface. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)1165-1168
    Number of pages3
    JournalJournal of Bacteriology
    Volume188
    Issue number3
    DOIs
    Publication statusPublished - Feb 2006

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