Nanocarriers for cytoplasmic delivery: Cellular uptake and intracellular fate of chitosan and hyaluronic acid-coated chitosan nanoparticles in a phagocytic cell model

Noha M. Zaki; Alessandro Nasti; Nicola Tirelli

doi:10.1002/mabi.201100156

Nanocarriers for cytoplasmic delivery: Cellular uptake and intracellular fate of chitosan and hyaluronic acid-coated chitosan nanoparticles in a phagocytic cell model

Noha M. Zaki, Alessandro Nasti, Nicola Tirelli

Research output: Contribution to journal › Article › peer-review

Abstract

The cellular uptake of hyaluronic-acid-coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non-phagocytic L929 fibroblasts as a control for uncoated nanoparticles. Both kinds of nanoparticles undergo endosomal escape and adopt a similar clathrin-based endocytic mechanism. The surface decoration with HA profoundly influences the kinetics of cellular uptake, with an at least two orders of magnitude slower kinetics, but also the nature of the binding on the cellular surface. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	1747-1760
Number of pages	13
Journal	Macromolecular Bioscience
Volume	11
Issue number	12
DOIs	https://doi.org/10.1002/mabi.201100156
Publication status	Published - 8 Dec 2011

Keywords

Chitosan
Clathrin
Endocytosis
Hyaluronic acid
Nanoparticles

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10.1002/mabi.201100156

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title = "Nanocarriers for cytoplasmic delivery: Cellular uptake and intracellular fate of chitosan and hyaluronic acid-coated chitosan nanoparticles in a phagocytic cell model",

abstract = "The cellular uptake of hyaluronic-acid-coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non-phagocytic L929 fibroblasts as a control for uncoated nanoparticles. Both kinds of nanoparticles undergo endosomal escape and adopt a similar clathrin-based endocytic mechanism. The surface decoration with HA profoundly influences the kinetics of cellular uptake, with an at least two orders of magnitude slower kinetics, but also the nature of the binding on the cellular surface. {\textcopyright} 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

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T1 - Nanocarriers for cytoplasmic delivery: Cellular uptake and intracellular fate of chitosan and hyaluronic acid-coated chitosan nanoparticles in a phagocytic cell model

AU - Zaki, Noha M.

AU - Nasti, Alessandro

AU - Tirelli, Nicola

PY - 2011/12/8

Y1 - 2011/12/8

N2 - The cellular uptake of hyaluronic-acid-coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non-phagocytic L929 fibroblasts as a control for uncoated nanoparticles. Both kinds of nanoparticles undergo endosomal escape and adopt a similar clathrin-based endocytic mechanism. The surface decoration with HA profoundly influences the kinetics of cellular uptake, with an at least two orders of magnitude slower kinetics, but also the nature of the binding on the cellular surface. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - The cellular uptake of hyaluronic-acid-coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non-phagocytic L929 fibroblasts as a control for uncoated nanoparticles. Both kinds of nanoparticles undergo endosomal escape and adopt a similar clathrin-based endocytic mechanism. The surface decoration with HA profoundly influences the kinetics of cellular uptake, with an at least two orders of magnitude slower kinetics, but also the nature of the binding on the cellular surface. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Chitosan

KW - Clathrin

KW - Endocytosis

KW - Hyaluronic acid

KW - Nanoparticles

U2 - 10.1002/mabi.201100156

DO - 10.1002/mabi.201100156

M3 - Article

SN - 1616-5195

VL - 11

SP - 1747

EP - 1760

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 12

ER -

Nanocarriers for cytoplasmic delivery: Cellular uptake and intracellular fate of chitosan and hyaluronic acid-coated chitosan nanoparticles in a phagocytic cell model

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Keywords

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