Nanoscale ligand spacing influences receptor triggering in T cells and NK cells

Derfogail Delcassian, David Depoil, Dominika Rudnicka, Mengling Liu, Daniel M. Davis, Michael L. Dustin, Iain E. Dunlop

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Bioactive nanoscale arrays were constructed to ligate activating cell surface receptors on T cells (the CD3 component of the TCR complex) and natural killer (NK) cells (CD16). These arrays are formed from biofunctionalized gold nanospheres with controlled interparticle spacing in the range 25-104 nm. Responses to these nanoarrays were assessed using the extent of membrane-localized phosphotyrosine in T cells stimulated with CD3-binding nanoarrays and the size of cell contact area for NK cells stimulated with CD16-binding nanoarrays. In both cases, the strength of response decreased with increasing spacing, falling to background levels by 69 nm in the T cell/anti-CD3 system and 104 nm for the NK cell/anti-CD16 system. These results demonstrate that immune receptor triggering can be influenced by the nanoscale spatial organization of receptor/ligand interactions. © 2013 American Chemical Society.
    Original languageEnglish
    Pages (from-to)5608-5614
    Number of pages6
    JournalNano Letters
    Volume13
    Issue number11
    DOIs
    Publication statusPublished - 13 Nov 2013

    Keywords

    • biofunctionalized nanoparticles
    • ligand density
    • Nanopatterning
    • NK cell
    • T cell

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