NAPO as a novel marker for apoptosis

Berna S. Sayan; Gulayse Ince; A. Emre Sayan; Mehmet Ozturk

doi:10.1083/jcb.200106044

NAPO as a novel marker for apoptosis

Berna S. Sayan, Gulayse Ince, A. Emre Sayan, Mehmet Ozturk

Research output: Contribution to journal › Article › peer-review

Abstract

Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody-defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.

Original language	English
Pages (from-to)	719-724
Number of pages	5
Journal	Journal of Cell Biology
Volume	155
Issue number	5
DOIs	https://doi.org/10.1083/jcb.200106044
Publication status	Published - 26 Nov 2001

Keywords

Apoptosis
Apoptotic cell death
Apoptotic marker
Quiescence
Senescence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "NAPO as a novel marker for apoptosis",

abstract = "Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody-defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.",

keywords = "Apoptosis, Apoptotic cell death, Apoptotic marker, Quiescence, Senescence",

author = "Sayan, {Berna S.} and Gulayse Ince and {Emre Sayan}, A. and Mehmet Ozturk",

note = "CA-54567, NCI NIH HHS, United States",

year = "2001",

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doi = "10.1083/jcb.200106044",

language = "English",

volume = "155",

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TY - JOUR

T1 - NAPO as a novel marker for apoptosis

AU - Sayan, Berna S.

AU - Ince, Gulayse

AU - Emre Sayan, A.

AU - Ozturk, Mehmet

N1 - CA-54567, NCI NIH HHS, United States

PY - 2001/11/26

Y1 - 2001/11/26

N2 - Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody-defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.

AB - Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody-defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.

KW - Apoptosis

KW - Apoptotic cell death

KW - Apoptotic marker

KW - Quiescence

KW - Senescence

U2 - 10.1083/jcb.200106044

DO - 10.1083/jcb.200106044

M3 - Article

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SN - 0021-9525

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SP - 719

EP - 724

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 5

ER -

NAPO as a novel marker for apoptosis

Abstract

Keywords

UN SDGs

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