TY - JOUR
T1 - Native chick laminin-4 containing the β2 chain (s-laminin) promotes motor axon growth
AU - Brandenberger, Ralph
AU - Kammerer, Richard A.
AU - Engel, Jürgen
AU - Chiquet, Matthias
PY - 1996
Y1 - 1996
N2 - After denervation of muscle, motor axons reinnervate original synaptic sites. A recombinant fragment of the synapse specific laminin β2 chain (s- laminin) was reported to inhibit motor axon growth. Consequently, a specific sequence (leucine-arginine-glutamate, LRE) of the laminin β2 chain was proposed to act as a stop signal and to mediate specific reinnervation at the neuromuscular junction (Porter, B.E., J. Weis, and J.R. Sanes. 1995. Neuron. 14:549-559). We demonstrate here that native chick laminin-4, which contains the β2 chain and is present in the synaptic basement membrane, does not inhibit but rather promotes motor axon growth. In native heterotrimeric laminin, the LRE sequence of the β2 chain is found in a triple coiled-coil region that is formed by all three subunits. We show here that the effect of LRE depends on the structural context. Whereas a recombinant randomly coiled LRE peptide indeed inhibited outgrowth by chick motoneurons, a small recombinant triple coiled- coil protein containing this sequence did not.
AB - After denervation of muscle, motor axons reinnervate original synaptic sites. A recombinant fragment of the synapse specific laminin β2 chain (s- laminin) was reported to inhibit motor axon growth. Consequently, a specific sequence (leucine-arginine-glutamate, LRE) of the laminin β2 chain was proposed to act as a stop signal and to mediate specific reinnervation at the neuromuscular junction (Porter, B.E., J. Weis, and J.R. Sanes. 1995. Neuron. 14:549-559). We demonstrate here that native chick laminin-4, which contains the β2 chain and is present in the synaptic basement membrane, does not inhibit but rather promotes motor axon growth. In native heterotrimeric laminin, the LRE sequence of the β2 chain is found in a triple coiled-coil region that is formed by all three subunits. We show here that the effect of LRE depends on the structural context. Whereas a recombinant randomly coiled LRE peptide indeed inhibited outgrowth by chick motoneurons, a small recombinant triple coiled- coil protein containing this sequence did not.
U2 - 10.1083/jcb.135.6.1583
DO - 10.1083/jcb.135.6.1583
M3 - Article
C2 - 8978824
SN - 0021-9525
VL - 135
SP - 1583
EP - 1592
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 6
ER -